Description

Sulforhodamine B CAS NO 3520-42-1 is a high-purity, water-soluble xanthene dye renowned for its intense fluorescence and excellent photostability. This compound is a critical reagent in advanced life science research and industrial applications, offering reliable performance and consistent quality. It is primarily utilized by researchers in biotechnology, pharmaceutical development, and analytical chemistry, as well as in specialized industrial processes requiring precise fluorescent labeling or detection.

Application

• Fluorescent Labeling and Staining: Widely used as a fluorescent tracer in cell biology for labeling proteins, nucleic acids, and cellular structures.
• In Vitro Diagnostics (IVD): Serves as a key component in the development of fluorescent immunoassays and diagnostic kits.
• Cytotoxicity Assays (SRB Assay): The standard dye for the Sulforhodamine B (SRB) assay, a colorimetric method for measuring cell proliferation and cytotoxicity in drug discovery.
• Flow Cytometry and Microscopy: Employed as a fluorescent probe in flow cytometry and fluorescence microscopy due to its bright emission and compatibility with common laser lines.
• Protein Binding Studies: Used to study protein-ligand interactions and protein quantification methods.
• Industrial and Material Science: Applied in the development of fluorescent sensors, security inks, and advanced materials.

Basic Information

Product Name	Sulforhodamine B
CAS No.	3520-42-1
Molecular Formula	C27H30N2O7S2
Molecular Weight	558.67 g/mol
Synonyms	Sulforhodamine B Acid Chloride; Lissamine Rhodamine B Sulfonyl Chloride; Acid Red 52; Sulforhodamine 101 acid chloride; SRB; Xylene Red B; Kiton Red 620; C.I. 45100; [9-(2-Carboxyphenyl)-6-diethylamino-3-xanthenylidene]-diethylammonium chloride hydrochloride
EINECS	222-529-8

Quality Control

Our Sulforhodamine B is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, spectrophotometry for absorbance/fluorescence verification, and identity confirmation (IR, NMR). We provide full traceability and Certificates of Analysis (COA) are available for every shipment, ensuring compliance with research-grade specifications and consistent batch-to-batch performance.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture.

Specification

Item	Specification
Appearance	Dark red to purple powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Absorbance (λ max)	565 - 575 nm
Emission (λ max)	585 - 595 nm
Loss on Drying	≤ 5.0 %
Solubility	Soluble in water, DMSO, methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.