Description

Pyrogallol Red is a synthetic triphenylmethane dye widely used as a sensitive and selective colorimetric reagent in analytical chemistry. Its primary value lies in forming stable, intensely colored complexes with various metal ions, enabling their detection and quantification. This compound is essential for researchers and quality control laboratories in the pharmaceutical, environmental, and material science sectors.

Application

• Spectrophotometric Determination of Proteins: Used in the Pyrogallol Red-Molybdate (PRM) assay for quantifying proteins in biological fluids, offering an alternative to the Bradford method.
• Metal Ion Analysis: Acts as a chromogenic agent for the colorimetric detection and determination of trace metals such as aluminum, gallium, and beryllium in water and alloy samples.
• Clinical Diagnostics: Employed in reagent formulations for automated clinical analyzers to measure serum albumin and other protein markers.
• Biochemical Research: Serves as a staining agent in gel electrophoresis and in studies of protein-binding interactions.
• Environmental Monitoring: Applied in test kits and procedures for assessing water quality through metal contamination analysis.
• Dye and Indicator: Functions as a pH indicator and as a colorant in specific histochemical staining protocols.

Basic Information

Product Name	Pyrogallol Red
CAS No.	3172-49-4
Molecular Formula	C19H12O8S
Molecular Weight	400.36 g/mol
Synonyms	Pyrogallolsulfonphthalein; 3,3',4',5,6,7-Hexahydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3-one, 6',6''-disulfonic acid; PGR; Pyrogallol Red Sodium Salt; C.I. 45435; Acid Red 114 (related class); Pyrogallolsulfonephthalein
EINECS	221-650-1

Quality Control

Our Pyrogallol Red is manufactured under a strict quality management system. Each batch undergoes comprehensive analytical testing, including identification by FT-IR and UV-Vis spectroscopy, purity assessment by HPLC, and control of moisture content. A Certificate of Analysis (COA) detailing all test results is provided to ensure compliance with your application requirements and internal specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated area at room temperature (15-25°C). Keep away from incompatible materials such as strong oxidizing agents.

Specification

Item	Specification
Appearance	Dark red to brownish-red powder
Identification (IR)	Conforms to reference spectrum
Identification (UV-Vis)	Conforms (λmax ~ 470-490 nm in suitable buffer)
Assay (HPLC)	≥ 95.0%
Loss on Drying	≤ 5.0 %
Residue on Ignition (Sulfated Ash)	≤ 1.0 %
Absorbance Ratio (A490/A260)	≥ 2.0 (Indicator of dye purity)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.