Description

7α-Hydroxy-4-Cholesten-3-One-D7 is a deuterium-labeled analog of a key intermediate in the bile acid biosynthesis pathway. This high-purity, stable isotope-labeled compound is essential for precise analytical quantification and metabolic pathway tracing in advanced research. It is primarily utilized by pharmaceutical R&D laboratories, clinical research organizations, and academic institutions focused on lipid metabolism, hepatology, and drug development studies.

Application

• Used as an internal standard in Liquid Chromatography-Mass Spectrometry (LC-MS) and Gas Chromatography-Mass Spectrometry (GC-MS) for the accurate quantification of endogenous 7α-hydroxy-4-cholesten-3-one in biological matrices.
• Critical for metabolic tracer studies investigating the cholesterol 7α-hydroxylase pathway and bile acid synthesis kinetics in vitro and in vivo.
• Facilitates drug discovery and development research targeting enzymes like CYP7A1 for therapeutic interventions in hypercholesterolemia and liver diseases.
• Enables clinical diagnostics research aimed at developing assays for biomarkers of bile acid deficiency or cholestatic liver disorders.
• Supports stable isotope dilution analysis (SIDA) methodologies to ensure high accuracy and reproducibility in complex sample analysis.

Basic Information

Item	Details
Product Name	7α-Hydroxy-4-Cholesten-3-One-D7
CAS No.	2260669-17-6
Molecular Formula	C27H37D7O2
Molecular Weight	~ 407.66 g/mol
Synonyms	7α-Hydroxy-4-cholesten-3-one-d7; 7α-Hydroxy-4-cholesten-3-one (7,7,8,8,11,12,12-d7); [D7]-7α-Hydroxy-4-cholesten-3-one; 7α-Hydroxy-5β-cholest-4-en-3-one-d7; Deuterated 7α-HC4; C4-d7; Stable Isotope Labeled Bile Acid Intermediate; Deuterium Labeled CYP7A1 Substrate
EINECS	Contact for details

Quality Control

Every batch of 7α-Hydroxy-4-Cholesten-3-One-D7 is manufactured and analyzed under strict quality systems to ensure exceptional isotopic purity and chemical integrity. Our products undergo rigorous quality testing, including advanced chromatographic and spectrometric methods, to ensure compliance with research-grade standards. A comprehensive Certificate of Analysis (COA) detailing isotopic enrichment, chemical purity, and identity confirmation is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation. For long-term storage, keep desiccated.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC-MS)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 98.0%
Isotopic Purity (MS)	≥ 98 atom % D
Chemical Purity	≥ 95%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.