Description

Propanoic Acid, 3-[2-[2-[2-[(4-Formylbenzoyl)Amino]Ethoxy]Ethoxy]Ethoxy]-, 2,5-Dioxo-1-Pyrrolidinyl Ester is a specialized heterobifunctional crosslinking reagent designed for advanced bioconjugation and material science applications. Its molecular architecture, featuring an activated NHS ester and a protected aldehyde group, provides a powerful and selective platform for coupling biomolecules under mild conditions. This compound is essential for researchers and manufacturers in pharmaceutical development, proteomics, and the creation of functionalized surfaces and hydrogels, where precise and stable covalent linkages are required.

Application

• Bioconjugation and Protein Labeling: Used to covalently link proteins, antibodies, or peptides to other molecules, surfaces, or carriers via amine-reactive NHS ester chemistry, with the aldehyde group available for further modification.
• Immobilization Chemistry: Functionalizes solid supports (e.g., beads, chips, plates) for creating affinity columns, biosensors, and diagnostic assay platforms.
• Polymer and Hydrogel Modification: Serves as a crosslinker or functional group introducer in the synthesis of smart polymers and biocompatible hydrogel networks.
• Antibody-Drug Conjugate (ADC) Research: A key intermediate in the development of linker-payload constructs for targeted cancer therapies.
• Peptide Synthesis: Employed as a building block or protecting group strategy in the synthesis of complex peptide constructs.
• Diagnostic Reagent Manufacturing: Used in the production of enzyme conjugates and labeled probes for ELISA, immunohistochemistry, and flow cytometry.

Basic Information

Product Name	Propanoic Acid, 3-[2-[2-[2-[(4-Formylbenzoyl)Amino]Ethoxy]Ethoxy]Ethoxy]-, 2,5-Dioxo-1-Pyrrolidinyl Ester
CAS No.	2101206-32-8
Molecular Formula	C23H28N2O9
Molecular Weight	476.48 g/mol
Synonyms	4-Formylbenzamide PEG3 NHS ester; NHS-PEG3-CHO; (4-Formylbenzoyl-PEG3)-NHS ester; 4-Formylbenzoic acid PEG3 NHS ester; N-(4-Formylbenzoyl)-3,6,9-trioxaundecanedioic acid 11-(2,5-dioxo-1-pyrrolidinyl) ester; 3,6,9-Trioxaundecanedioic acid, 11-[(4-formylbenzoyl)amino]-1-[(2,5-dioxo-1-pyrrolidinyl) ester]; Heterobifunctional PEG crosslinker, NHS-PEG3-aldehyde
EINECS	Contact for details

Quality Control

Our production of this high-purity biochemical reagent adheres to stringent ISO 9001 quality management systems. Every batch is characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity, purity, and functional integrity. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with each shipment to ensure traceability and support your research and quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and the active ester moiety is susceptible to hydrolysis. For long-term stability, store under an inert atmosphere. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay	≥ 90% (Functional NHS ester)
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.