Description

Glycoursodeoxycholic-2,2,4,4-D4 Acid CAS NO 2044276-17-5 is a deuterium-labeled derivative of the primary bile acid glycoursodeoxycholic acid (GUDCA), a key metabolite of ursodeoxycholic acid (UDCA). This stable isotope-labeled compound is essential for use as an internal standard in quantitative mass spectrometry-based bioanalytical assays, ensuring precise and accurate measurement of endogenous bile acid levels in complex biological matrices. It is a critical tool for researchers and quality control laboratories in the pharmaceutical, clinical diagnostics, and metabolomics fields, where reliable quantification is paramount for drug development, disease biomarker discovery, and pharmacokinetic studies.

Application

• Internal Standard for LC-MS/MS Analysis: Primary application for the accurate quantification of glycoursodeoxycholic acid and related bile acids in plasma, serum, urine, and tissue samples.
• Pharmaceutical R&D and Bioanalysis: Used in pharmacokinetic (PK) and pharmacodynamic (PD) studies of bile acid-based therapeutics and metabolic drugs.
• Clinical Diagnostics and Metabolomics: Enables precise profiling of bile acid metabolism for research into liver diseases (cholestasis, PBC, PSC), metabolic disorders, and gut microbiome interactions.
• Drug Metabolism and Disposition Studies (DMPK): Critical for tracing the metabolic fate of deuterated drug candidates and understanding metabolic pathways.
• Quality Control and Method Validation: Serves as a certified reference material for validating analytical methods in compliance with GLP and GMP guidelines.
• Nutritional and Food Science Research: Applied in studies investigating the impact of diet on bile acid metabolism and gut health.

Basic Information

Product Name	Glycoursodeoxycholic-2,2,4,4-D4 Acid
CAS No.	2044276-17-5
Molecular Formula	C₂₆H₄₁D₄NO₆
Molecular Weight	~471.66 g/mol
Synonyms	GUDCA-d4; Glycoursodeoxycholic Acid-d4; N-[(3α,5β,7β)-3,7-Dihydroxy-24-oxocholan-24-yl-d4]glycine; Ursodeoxycholylglycine-d4; Deuterated Glycoursodeoxycholic Acid; [2,2,4,4-D4]-Glycoursodeoxycholic Acid; Cholylglycine-d4 (specific isomer); Tauro-ursodeoxycholic acid-d4 analog
EINECS	Contact for details

Quality Control

Our deuterated standards are manufactured under strict quality systems to ensure the high chemical purity and isotopic enrichment required for sensitive analytical applications. Each batch is characterized by advanced spectroscopic and chromatographic techniques, including NMR, HPLC, and mass spectrometry. A comprehensive Certificate of Analysis (COA) is provided, detailing purity (by HPLC), isotopic purity (by MS), and chemical identity (by 1H-NMR). Production follows relevant ISO guidelines, and materials are suitable for use in GLP/GMP-compliant laboratories.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term storage, keep under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to expected mass spectrum
Purity (HPLC)	≥ 98.0%
Isotopic Purity	≥ 98 atom % D
Chemical Purity (NMR)	Conforms to structure
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.