Description

Glycolithocholic-2,2,4,4-D4 Acid is a high-purity, deuterium-labeled derivative of glycolithocholic acid, a key bile acid metabolite. This stable isotope-labeled compound is essential for providing an internal standard in quantitative mass spectrometry, ensuring unparalleled accuracy and reproducibility in analytical workflows. It is primarily required by research institutions, pharmaceutical developers, and CROs engaged in metabolomics, lipidomics, and pharmacokinetic studies related to liver function and bile acid metabolism.

Application

• Internal Standard for LC-MS/MS: Critical for the precise quantification of endogenous glycolithocholic acid and related bile acids in biological matrices like serum, plasma, and urine.
• Metabolomics Research: Enables accurate profiling of bile acid pathways in studies of liver diseases, cholestasis, and metabolic disorders.
• Drug Metabolism and Pharmacokinetics (DMPK): Used as a tracer to study the absorption, distribution, metabolism, and excretion (ADME) of bile acid-based therapeutics.
• Diagnostic Assay Development: Serves as a reference standard in the development and validation of clinical diagnostic tests for hepatobiliary health.
• Biochemical Research: A tool for investigating the role of specific bile acids in cellular signaling, gut microbiome interactions, and metabolic regulation.

Basic Information

Product Name	Glycolithocholic-2,2,4,4-D4 Acid
CAS No.	2044276-16-4
Molecular Formula	C₂₆H₄₀D₄O₅
Molecular Weight	~448.64 g/mol
Synonyms	3α-Hydroxy-5β-cholan-24-oic Acid 3-Glucuronide-2,2,4,4-D4; Glycolithocholic Acid-d4; Lithocholic Acid 3-O-Glucuronide-d4; 3α-Hydroxy-5β-cholanic Acid-24-Glucuronide-d4; N-[(3α,5β)-3-Hydroxy-24-oxocholan-24-yl]-β-D-Glucopyranosiduronic Acid-d4; Deuterated Glycolithocholic Acid; GLC-d4; LCA-3G-d4
EINECS	Contact for details

Quality Control

Every batch of Glycolithocholic-2,2,4,4-D4 Acid CAS NO 2044276-16-4 is manufactured and analyzed under strict quality systems. We guarantee high chemical and isotopic purity, verified by advanced analytical techniques including NMR, HPLC, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, isotopic enrichment, and solvent content is provided with each shipment to ensure traceability and compliance with your research or development protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere for long-term stability. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 98 atom % D
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.