Description

2,5-Dihydroxybenzoic Acid-D3 is a deuterium-labeled isotopologue of gentisic acid, a key metabolite and analytical standard. This high-purity compound is essential for applications requiring precise internal standards in mass spectrometry, enabling accurate quantification and eliminating matrix effects. It is primarily used by researchers and quality control laboratories in the pharmaceutical, biotechnology, and clinical diagnostics sectors for advanced analytical method development and validation.

Application

• Internal Standard for LC-MS/MS: Serves as a critical deuterated internal standard for the precise quantification of gentisic acid and related phenolic compounds in complex biological matrices like plasma, urine, and tissue homogenates.
• Metabolomics & Biomarker Research: Used in stable isotope dilution assays to study metabolic pathways, oxidative stress markers, and disease biomarkers with high accuracy and reproducibility.
• Pharmaceutical Analysis: Enables reliable pharmacokinetic (PK) and pharmacodynamic (PD) studies of drug candidates and their metabolites in preclinical and clinical research.
• Food & Nutraceutical Testing: Applied in the analysis of natural products, herbal extracts, and functional foods for the quantification of salicylate derivatives and antioxidants.
• Environmental Analysis: Facilitates the trace-level detection and measurement of phenolic acid pollutants in environmental samples.
• Method Development & Validation: A key tool for developing, optimizing, and validating robust quantitative analytical methods in compliance with regulatory guidelines (e.g., FDA, ICH).

Basic Information

Product Name	2,5-Dihydroxybenzoic Acid-D3
CAS No.	294661-02-2
Molecular Formula	C7H3D3O4
Molecular Weight	157.12 g/mol
Synonyms	Gentisic Acid-d3; 2,5-Dihydroxybenzoic-2,4,6-d3 Acid; 2,5-Dihydroxy-d3-benzoic Acid; 5-Hydroxysalicylic Acid-d3; Deuterated Gentisic Acid; [2H3]-2,5-Dihydroxybenzoic Acid; 2,4,6-Tri-deutero-2,5-dihydroxybenzoic Acid
EINECS	Contact for details

Quality Control

Every batch of 2,5-Dihydroxybenzoic Acid-D3 is manufactured and tested under strict quality management systems. We guarantee high chemical and isotopic purity, verified by advanced analytical techniques including NMR, HPLC, and Mass Spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, isotopic enrichment, and analytical results is provided with each shipment to ensure compliance with your research and quality standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to minimize exposure to atmospheric humidity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (NMR/MS)	≥ 98.0 atom % D
Chemical Purity	≥ 98.0%
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.