Description

(1R,2S)-(-)-Ephedrine-D3 Hcl (n-Methyl-D3) is a high-purity, deuterium-labeled isotopologue of the classic sympathomimetic amine, ephedrine. This compound is specifically designed for use as an internal standard in quantitative analytical methods, providing unmatched accuracy and reliability in mass spectrometry and other detection techniques. It is an essential tool for researchers and quality control laboratories in the pharmaceutical, forensic, and clinical diagnostics sectors who require precise measurement of ephedrine and related compounds.

Application

• Internal Standard for LC-MS/MS and GC-MS: Primary application for the accurate quantification of ephedrine, pseudoephedrine, and related alkaloids in complex biological matrices.
• Pharmaceutical Metabolism and Pharmacokinetic (PK/ADME) Studies: Used to trace and quantify the metabolic fate of ephedrine in vivo without isotopic interference.
• Forensic and Anti-Doping Analysis: Critical for developing and validating highly specific and sensitive methods to detect stimulants in compliance with WADA and other regulatory guidelines.
• Clinical Research and Biomarker Validation: Enables precise measurement of endogenous or administered ephedrine levels in plasma, urine, and tissue samples.
• Reference Material for Method Development: Serves as a certified reference material to calibrate instruments and validate new analytical procedures in QA/QC labs.
• Stable Isotope Labeling in Chemical Synthesis: Used as a building block in synthetic chemistry for mechanistic studies or to create other labeled compounds.

Basic Information

Product Name	(1R,2S)-(-)-Ephedrine-D3 Hcl (n-Methyl-D3)
CAS No.	285979-72-8
Molecular Formula	C10H12D3NO•HCl
Molecular Weight	204.73 g/mol (approx.)
Synonyms	(-)-Ephedrine-d3 (N-methyl-d3) hydrochloride; (1R,2S)-1-Phenyl-2-(methyl-d3-amino)propan-1-ol hydrochloride; l-Ephedrine-d3 HCl; [N-methyl-d3]-Ephedrine hydrochloride; Deuterated Ephedrine HCl; Ephedrine-N-CD3 hydrochloride; Isotopic Internal Standard EPI-d3; 1R,2S-(-)-Ephedrine-N-methyl-d3 hydrochloride
EINECS	Contact for details

Quality Control

Every batch of (1R,2S)-(-)-Ephedrine-D3 Hcl (n-Methyl-D3) is manufactured and analyzed under strict quality systems to ensure the highest isotopic and chemical purity for critical analytical applications. Our products undergo rigorous quality testing, including NMR, HPLC, and MS, to confirm identity, purity, and isotopic enrichment. Certificates of Analysis (COA) detailing batch-specific results are provided and traceable to certified reference materials.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry, cool place. This material is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (deuterium incorporation)	≥ 98 atom % D (N-methyl position)
Chemical Purity (by HPLC)	≥ 99.0%
Specific Rotation [α]D20	-33° to -36° (c=1 in H2O)
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.