Description

Choline-D9 Bromide (N,N,n-Trimethyl-D9) is a stable isotope-labeled analog of choline bromide, where nine hydrogen atoms are replaced by deuterium. This high-purity compound is essential for use as an internal standard in quantitative mass spectrometry, ensuring unparalleled accuracy in analytical measurements. It is critically needed by researchers and quality control laboratories in the pharmaceutical, nutritional science, and metabolomics fields for precise quantification of choline and related metabolites.

Application

• Quantitative Mass Spectrometry (LC-MS/MS, GC-MS): Serves as a critical internal standard for the accurate quantification of choline, acetylcholine, and phosphatidylcholine in complex biological matrices.
• Pharmaceutical R&D and Bioanalysis: Used in pharmacokinetic (PK) and pharmacodynamic (PD) studies to track drug metabolism and biomarker discovery related to cholinergic pathways.
• Nutritional and Clinical Research: Enables precise measurement of choline uptake, metabolism, and status in human and animal studies, supporting research on liver health, brain function, and prenatal nutrition.
• Metabolomics and Biomarker Validation: Provides a stable isotope reference for high-confidence identification and quantification of choline-containing metabolites in profiling studies.
• Quality Control in Dietary Supplements: Employed in assay development and validation for the accurate determination of choline content in fortified foods and supplement formulations.
• Neuroscience Research: Facilitates precise analysis of cholinergic neurotransmission and related biochemical processes in neurological disease models.

Basic Information

Product Name	Choline-D9 Bromide (N,N,n-Trimethyl-D9)
CAS No.	285979-71-7
Molecular Formula	C5D9BrNO
Molecular Weight	187.10 g/mol (for deuterated form)
Synonyms	(2-Hydroxyethyl)trimethyl-d9-ammonium Bromide; Choline-d9 Bromide; Deuterated Choline Bromide; (2-Hydroxyethyl-d4)trimethyl-d9-ammonium Bromide; N,N,N-Trimethyl-d9-2-hydroxyethanaminium Bromide; (β-Hydroxyethyl)trimethylammonium-d9 Bromide; Choline Bromide-d9; 2-Hydroxy-N,N,N-trimethyl-d9-ethanaminium Bromide
EINECS	Contact for details

Quality Control

Our Choline-D9 Bromide is manufactured under strict quality systems to ensure the high chemical and isotopic purity required for analytical standards. Each batch is characterized by advanced spectroscopic and chromatographic techniques, including NMR, HPLC, and mass spectrometry, to confirm identity, isotopic enrichment (typically ≥98 atom % D), and chemical purity (typically ≥95%). A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This compound is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and purity. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Isotopic Enrichment (MS)	≥ 98 atom % D
Chemical Purity (NMR)	≥ 95%
Water Content (KF)	≤ 2.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.