Description

Choline-1,1,2,2-D4 Chloride is a stable isotope-labeled analog of choline chloride, where four hydrogen atoms are replaced by deuterium. This compound is essential as an internal standard in quantitative mass spectrometry, enabling precise and accurate measurement of choline and its metabolites in complex biological matrices. It is primarily utilized by researchers and analytical laboratories in the fields of pharmaceutical development, nutritional science, and clinical diagnostics for method validation and high-fidelity biomarker analysis.

Application

• Internal Standard for LC-MS/MS: Critical for the quantitative analysis of choline, acetylcholine, and related metabolites in pharmacokinetic and metabolomic studies.
• Biomedical Research: Used in tracer studies to investigate choline metabolism, uptake, and phospholipid synthesis pathways.
• Nutrient Fortification Studies: Enables precise tracking and quantification of choline in fortified foods and dietary supplements.
• Clinical Diagnostics: Supports the development of robust assays for measuring choline levels in plasma, serum, and other bodily fluids for disease research.
• Pharmaceutical Quality Control: Employed in the analysis of drug formulations and active pharmaceutical ingredients (APIs) containing choline.
• Neuroscience Research: Facilitates studies on cholinergic neurotransmission and brain function using stable isotope labeling techniques.

Basic Information

Item	Details
Product Name	Choline-1,1,2,2-D4 Chloride
CAS No.	285979-70-6
Molecular Formula	C5H10D4ClNO
Molecular Weight	143.65 g/mol (for D4 labeled form)
Synonyms	(2-Hydroxyethyl)trimethylammonium-1,1,2,2-d4 Chloride; Choline-d4 Chloride; Deuterocholine Chloride; 2-Hydroxy-N,N,N-trimethylethanaminium-1,1,2,2-d4 Chloride; Biocolina-d4; Hepacholine-d4; (β-Hydroxyethyl)trimethylammonium-d4 Chloride; Lipotril-d4
EINECS	Contact for details

Quality Control

Our Choline-1,1,2,2-D4 Chloride is manufactured under strict quality control protocols to ensure high isotopic purity and chemical integrity, suitable for sensitive analytical applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing parameters such as isotopic enrichment (D4 content), chemical purity, and absence of interfering impurities. We adhere to cGMP principles for consistent quality, supporting research that demands reliable and traceable reference materials.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic nature, the container must be kept tightly sealed in a dry environment to prevent moisture absorption, which can affect stability and analytical performance. For long-term storage, consider desiccants or inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment (MS)	≥ 98 atom % D
Chemical Purity (NMR)	Conforms
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.