Description

(S)-(-)-Nicotine-D4 CAS NO 284685-07-0 is a deuterium-labeled isotopologue of the natural alkaloid (S)-nicotine, where four hydrogen atoms are replaced by deuterium. This stable isotope-labeled compound is an essential internal standard for the precise quantification and metabolic profiling of nicotine in complex biological matrices. It is primarily required by analytical laboratories, contract research organizations (CROs), and pharmaceutical companies engaged in advanced LC-MS/MS and GC-MS research related to tobacco science, pharmacokinetics, and forensic toxicology.

Application

• Quantitative Internal Standard for LC-MS/MS & GC-MS: Critical for achieving high accuracy and precision in the bioanalysis of nicotine and its metabolites in plasma, urine, and tissue samples.
• Metabolic and Pharmacokinetic (PK/PD) Studies: Enables tracking and differentiation of administered nicotine from endogenous sources in ADME (Absorption, Distribution, Metabolism, Excretion) research.
• Forensic and Clinical Toxicology: Used as a reference standard in method development and validation for the confirmatory testing of nicotine exposure.
• Tobacco Product Research: Supports analytical studies on nicotine uptake, delivery, and behavioral effects from cigarettes, e-liquids, and nicotine replacement therapies (NRTs).
• Isotope Dilution Mass Spectrometry (IDMS): Serves as the spike material for this gold-standard technique, ensuring minimal analytical error in certification of reference materials.
• Environmental and Occupational Exposure Monitoring: Facilitates sensitive detection of trace nicotine levels in air, dust, and surface wipe samples.

Basic Information

Product Name	(S)-(-)-Nicotine-D4
CAS No.	284685-07-0
Molecular Formula	C₁₀H₁₀D₄N₂
Molecular Weight	166.26 g/mol
Synonyms	(-)-Nicotine-d4; (S)-Nicotine-d4; (S)-3-(1-Methyl-2-pyrrolidinyl)pyridine-d4; Deuterated Nicotine; Nicotine-2,4,5,6-d4; Nicotine-d4 Internal Standard; Nicotine Isotopologue; [2,4,5,6-²H₄]Nicotine
EINECS	Contact for details

Quality Control

Every batch of our (S)-(-)-Nicotine-D4 is manufactured and analyzed under strict quality systems. We provide comprehensive analytical data to support its use in regulated environments. The product is characterized by NMR, HPLC, and MS to confirm chemical structure, isotopic purity (D-enrichment), and chemical purity. A detailed Certificate of Analysis (COA) is supplied with each shipment, reporting batch-specific results for identity, assay, and impurity profile.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C under an inert atmosphere (e.g., argon or nitrogen) to prevent oxidation. For long-term storage, keep desiccated at -20°C. The compound is light-sensitive and easily oxidized; handle in a well-ventilated fume hood using appropriate personal protective equipment.

Specification

Item	Specification
Appearance	Colorless to pale yellow liquid
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D-enrichment)	≥ 98 atom % D
Chemical Purity (by GC)	≥ 99.0%
Specific Optical Rotation	-160° to -168° (c=2, ethanol)
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.