Description

L-Lysine-4,4,5,5-D4 Hcl is a stable isotope-labeled derivative of the essential amino acid L-Lysine, specifically deuterated at the 4,4,5,5 positions of the carbon chain. This compound is crucial for use as an internal standard in quantitative mass spectrometry, ensuring unparalleled accuracy and reliability in analytical measurements. It is primarily required by research institutions, pharmaceutical companies, and CROs (Contract Research Organizations) engaged in metabolomics, pharmacokinetic studies, and proteomic research where precise quantification is non-negotiable.

Application

• Quantitative Mass Spectrometry (MS) & LC-MS/MS: Serves as a critical internal standard for the accurate quantification of endogenous L-Lysine in complex biological matrices like plasma, urine, and tissue homogenates.
• Metabolomics & Biomarker Discovery: Enables precise tracking of lysine metabolism and flux analysis in metabolic pathway studies to identify disease biomarkers.
• Pharmacokinetic/Pharmacodynamic (PK/PD) Studies: Used in drug development to monitor the absorption, distribution, metabolism, and excretion of drug candidates or nutrients.
• Proteomics & Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC): Facilitates accurate protein quantification and turnover rate studies in cell-based research.
• Clinical Diagnostics: Supports the development and validation of high-precision diagnostic assays for inborn errors of metabolism related to lysine.
• Nutritional Research: Employed in studies investigating protein digestion, amino acid bioavailability, and nutritional status assessment.
• Forensic & Doping Control Analysis: Provides a reliable reference standard for confirmatory testing in forensic toxicology and anti-doping programs.

Basic Information

Product Name	L-Lysine-4,4,5,5-D4 Hcl
CAS No.	284664-96-6
Molecular Formula	C6H10D4ClN2O2
Molecular Weight	183.67 g/mol (for monoisotopic)
Synonyms	(2S)-2,6-Diamino-4,4,5,5-tetradeuteriohexanoic acid hydrochloride; L-Lysine-d4 hydrochloride; L-Lysine-4,4,5,5-d4 hydrochloride; (S)-2,6-Diaminocaproic-4,4,5,5-d4 acid hydrochloride; Deuterated L-Lysine HCl; Lysine-(4,5,5,5-D4) hydrochloride; Stable Isotope Labeled L-Lysine HCl; Internal Standard for Lysine Quantification
EINECS	Contact for details

Quality Control

Every batch of L-Lysine-4,4,5,5-D4 Hcl is manufactured and analyzed under strict quality systems to ensure the high chemical purity and isotopic enrichment required for sensitive analytical applications. We provide comprehensive Certificates of Analysis (COA) that detail specifications for isotopic purity (atom % D), chemical purity (by HPLC), and identification. Our quality commitment aligns with the standards expected in pharmaceutical and high-end research environments.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation. Keep container tightly sealed in a dry and well-ventilated place.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure and deuteration pattern
Purity (HPLC)	≥ 98.0%
Isotopic Purity (Atom % D)	≥ 98.0% (by MS)
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Specific Optical Rotation	+19.0° to +21.5° (c=2 in 6N HCl)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.