Description

D-Leucine-D10 is a stable isotope-labeled analog of the essential amino acid L-leucine, where ten hydrogen atoms are replaced by deuterium. This high level of deuteration makes it an invaluable internal standard for quantitative mass spectrometry, ensuring superior accuracy and precision in analytical measurements. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors for advanced metabolic studies, proteomics, and drug development.

Application

• Quantitative Mass Spectrometry (MS): Serves as a critical internal standard for the precise quantification of endogenous leucine and related metabolites in complex biological matrices like plasma, urine, and tissue samples.
• Metabolic Pathway Analysis: Used in tracer studies to investigate protein synthesis rates, branched-chain amino acid (BCAA) metabolism, and metabolic disorders using techniques like GC-MS or LC-MS/MS.
• Pharmaceutical R&D: Essential in pharmacokinetic (PK) and pharmacodynamic (PD) studies for the development of new therapeutic agents, enabling accurate measurement of drug metabolites.
• Proteomics and Biomarker Discovery: Facilitates accurate relative and absolute quantification (AQUA) of peptides and proteins in shotgun proteomics workflows.
• Clinical Diagnostics: Employed in the development and validation of high-precision clinical assays for inborn errors of metabolism, such as maple syrup urine disease (MSUD).
• Nutritional Research: Used to study leucine turnover, muscle protein synthesis, and the metabolic effects of dietary supplements in sports science and aging research.

Basic Information

Product Name	D-Leucine-D10
CAS No.	271247-12-2
Molecular Formula	C6D10H3NO2
Molecular Weight	137.20 g/mol (for deuterated form)
Synonyms	(2R)-2-Amino-4-methylpentanoic-3,3,4,4,5,5,5-d7-2-d3 acid; Deuterated D-Leucine; D-Leucine-d10; (R)-2-Amino-4-methylpentanoic-d10 acid; Leucine-d10 (D-isomer); (R)-Leucine-d10; (2R)-2-Azaniumyl-4-methylpentanoate-d10; Stable Isotope Labeled D-Leucine
EINECS	Contact for details

Quality Control

Our D-Leucine-D10 is manufactured under strict quality systems to ensure the highest isotopic and chemical purity required for sensitive analytical applications. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing parameters such as isotopic purity (atom % D), chemical purity (HPLC), and chiral purity. We adhere to relevant industry standards for high-purity reference materials.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation and storage under an inert atmosphere to maintain stability and isotopic integrity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (Atom % D)	≥ 98.0%
Chiral Purity (Optical Rotation)	≥ 99.0% D-isomer
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.