Description

Phenolphthalein Diphosphate Pyridine Salt is a high-purity, chromogenic substrate specifically designed for enzymatic detection and quantification. This compound is critical for biochemical assays where precise and sensitive measurement of alkaline phosphatase activity is required. It is primarily utilized by research institutions, diagnostic kit manufacturers, and quality control laboratories in the life sciences and pharmaceutical sectors.

Application

• Enzymatic Substrate for Alkaline Phosphatase (ALP): Serves as a key chromogenic reagent in ELISA, immunoblotting, and other immunoassay platforms for detecting ALP-conjugated antibodies.
• Clinical Diagnostics: Used in the formulation of diagnostic kits for measuring ALP levels, an important biomarker for liver and bone disorders.
• Molecular Biology Research: Employed in nucleic acid detection systems (e.g., Southern, Northern, and Western blots) that utilize ALP-based colorimetric detection.
• Histochemistry & Cytochemistry: Applied as a substrate for visualizing ALP activity in tissue sections and cell cultures.
• Food Safety & Environmental Testing: Incorporated into biosensor and assay systems for detecting pathogens or contaminants via enzyme-linked methods.
• Pharmaceutical Quality Control: Used in potency assays and stability testing for biopharmaceuticals that employ ALP tags or related enzymatic processes.

Basic Information

Product Name	Phenolphthalein Diphosphate Pyridine Salt
CAS No.	267240-23-3
Molecular Formula	C31H25N2O8P
Molecular Weight	584.51 g/mol
Synonyms	Phenolphthalein Diphosphate Monopyridinium Salt; Phenolphthalein Diphosphate, Pyridine Salt; Phenolphthalein-3',3''-bis(phosphate) Pyridinium Salt; PPP; Phenolphthalein-3,3-bisphosphate Pyridinium Salt; ALP Substrate; 3,3-Bis(4-phosphonoxyphenyl)-1(3H)-isobenzofuranone Pyridine Salt
EINECS	Contact for details

Quality Control

Our Phenolphthalein Diphosphate Pyridine Salt is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for critical diagnostic and research applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under dry conditions to maintain stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMSO
Functional Activity	Suitable for enzyme assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.