Description

L-Alanine-2,3,3,3-D4-n-Fmoc is a deuterium-labeled, N-Fmoc protected derivative of the amino acid alanine, specifically designed for advanced research and development. This compound is essential for applications requiring isotopic labeling, such as metabolic pathway tracing and mass spectrometry-based quantification, providing critical insights with minimal background interference. It is primarily utilized by pharmaceutical researchers, analytical chemists, and biochemists in drug discovery, proteomics, and metabolic studies where precise tracking and high-purity building blocks are paramount.

Application

• Isotope-Labeled Peptide Synthesis: Serves as a deuterated building block in solid-phase peptide synthesis (SPPS) for creating labeled peptides used in mass spectrometry.
• Metabolic Research & Tracer Studies: Used to investigate amino acid metabolism, protein turnover, and biochemical pathways in vivo and in vitro.
• Internal Standards for Quantitative Analysis: Acts as a high-purity internal standard in LC-MS/MS and GC-MS methods for the accurate quantification of alanine and related compounds.
• Structural Biology & NMR Spectroscopy: The deuterium labels aid in protein structural analysis by simplifying complex NMR spectra.
• Pharmaceutical R&D: Employed in the discovery and development of new therapeutics, particularly for studying drug metabolism and pharmacokinetics (DMPK).
• Proteomics Research: Facilitates relative and absolute quantification (e.g., in SILAC or AQUA strategies) to study protein expression and post-translational modifications.

Basic Information

Product Name	L-Alanine-2,3,3,3-D4-n-Fmoc
CAS No.	225101-69-9
Molecular Formula	C18H13D4NO4
Molecular Weight	315.35 g/mol
Synonyms	Fmoc-L-alanine-2,3,3,3-d4; Fmoc-Ala(D4)-OH; N-[(9H-Fluoren-9-ylmethoxy)carbonyl]-L-alanine-2,3,3,3-d4; (2S)-2-[(9H-Fluoren-9-ylmethoxy)carbonylamino]propanoic-2,3,3,3-d4 acid; Fmoc-L-Ala(D4)-OH; Deuterated Fmoc-L-alanine; Fmoc-protected deuterated alanine; L-Alanine-2,3,3,3-tetradeutero-N-[(9H-fluoren-9-ylmethoxy)carbonyl]
EINECS	Contact for details

Quality Control

Every batch of L-Alanine-2,3,3,3-D4-n-Fmoc is manufactured and tested under strict quality management systems to ensure the highest standards of isotopic purity and chemical integrity. Our products undergo rigorous quality testing, including advanced chromatographic and spectroscopic methods, to ensure compliance with research-grade specifications. Certificates of Analysis (COA) detailing isotopic enrichment, chemical purity, and identity are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This material is hygroscopic (moisture-sensitive) and must be kept under dry, inert conditions. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 98 atom % D
Optical Rotation	[α]D20: -15.0° to -20.0° (c=1 in DMF)
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.