Description

L-Alanine-2-D1-n-Fmoc is a deuterium-labeled derivative of the amino acid alanine, protected at the amine terminus with a 9-fluorenylmethoxycarbonyl (Fmoc) group. This compound is a critical building block in the synthesis of isotopically labeled peptides and proteins for advanced research applications, including metabolic studies, NMR spectroscopy, and mass spectrometry. It is primarily utilized by pharmaceutical R&D departments, academic research institutions, and CROs specializing in proteomics and drug discovery.

Application

• Deuterium-Labeled Peptide Synthesis: Essential for the solid-phase and solution-phase synthesis of peptides containing stable isotope labels for structural and mechanistic studies.
• Metabolic Pathway Tracing: Used to incorporate deuterium tracers into biomolecules to study metabolic flux, protein turnover, and degradation pathways in vivo and in vitro.
• NMR Spectroscopy: Serves as a precursor for preparing isotopically enriched proteins to simplify complex NMR spectra and facilitate three-dimensional structure determination.
• Mass Spectrometry Standards: Employed in the preparation of internal standards for quantitative proteomics and pharmacokinetic studies, enhancing analytical accuracy and reproducibility.
• Proteomics Research: A key reagent for labeling strategies (e.g., SILAC) to enable comparative quantitative analysis of protein expression levels.
• Bioconjugation & Probe Development: Used in the creation of labeled probes for imaging, biomarker discovery, and target engagement studies.

Basic Information

Product Name	L-Alanine-2-D1-n-Fmoc
CAS No.	225101-64-4
Molecular Formula	C18H16DNO4
Molecular Weight	311.34 g/mol
Synonyms	Fmoc-L-alanine-2-d1; Fmoc-Ala-2-d1-OH; (2S)-2-[(9H-Fluoren-9-ylmethoxy)carbonylamino]propanoic-2-d1 acid; N-[(9H-Fluoren-9-ylmethoxy)carbonyl]-L-alanine-2-d1; N-Fmoc-L-alanine-2-d1; Deuterated Fmoc-L-alanine; Fmoc-protected deuterated alanine; L-Alanine-2-d1, N-[(9H-fluoren-9-ylmethoxy)carbonyl]-
EINECS	Contact for details

Quality Control

Our L-Alanine-2-D1-n-Fmoc is manufactured under strict quality systems to ensure high isotopic purity and chemical integrity. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing parameters such as chemical purity (by HPLC), isotopic enrichment (by NMR/MS), chiral purity, and moisture content. We adhere to cGMP guidelines for advanced pharmaceutical intermediates, ensuring traceability and consistency for critical research applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (NMR/MS)	≥ 98 atom % D
Chiral Purity	≥ 99.0% (L-isomer)
Water Content (KF)	≤ 0.5%
Heavy Metals	≤ 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.