Description

3-n-Maleimidobenzohydrazide-Hcl CAS NO 223528-57-2 is a specialized heterobifunctional crosslinking reagent featuring a maleimide group and a hydrazide moiety. This compound is critical for creating stable, site-specific conjugates between biomolecules, such as antibodies, peptides, and oligonucleotides, under mild aqueous conditions. It is an essential tool for researchers and manufacturers in the fields of bioconjugation, diagnostic assay development, and targeted drug delivery systems.

Application

• Site-specific bioconjugation of antibodies and antibody-drug conjugates (ADCs).
• Crosslinking agent for peptide and protein modification in therapeutic development.
• Preparation of enzyme and fluorescent probe conjugates for diagnostic assays.
• Surface functionalization of nanoparticles and polymers for targeted delivery platforms.
• Linker chemistry in the synthesis of immunogens and vaccine components.
• Tool compound for chemical biology research involving protein labeling and tracking.

Basic Information

Product Name	3-n-Maleimidobenzohydrazide-Hcl
CAS No.	223528-57-2
Molecular Formula	C11H10N4O3 • HCl
Molecular Weight	282.68 g/mol (Free base: 246.22 g/mol)
Synonyms	3-(Maleimidobenzoyl)hydrazide hydrochloride; 3-Maleimidobenzhydrazide HCl; 3-Maleimidobenzoic acid hydrazide hydrochloride; MBH-HCl; 3-Maleimidobenzoyl hydrazine hydrochloride; N-(3-Hydrazinyl-3-oxopropyl)maleimide hydrochloride; 3-(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)benzhydrazide hydrochloride
EINECS	Contact for details

Quality Control

Our 3-n-Maleimidobenzohydrazide-Hcl is manufactured under strict quality management systems to ensure high purity and batch-to-batch consistency, suitable for research and biopharmaceutical applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant quality guidelines to support our customers' regulatory and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as indicated on the label or COA. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Single Unknown Impurity (HPLC)	≤ 0.5%
Total Impurities (HPLC)	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.