Description

3,3,3-Trideuterio-2-Hydroxy-n-[4-Nitro-3-(Trifluoromethyl)Phenyl]-2-(Trideuteriomethyl)Propanamide is a deuterated isotopologue of a key pharmaceutical intermediate. This compound is specifically designed for use in drug metabolism and pharmacokinetic (DMPK) studies, providing critical insights into the absorption, distribution, metabolism, and excretion of active pharmaceutical ingredients. It is an essential tool for researchers and developers in the pharmaceutical, agrochemical, and life science industries who require high-purity, stable-labeled internal standards for mass spectrometry and analytical method development.

Application

• Internal Standard for LC-MS/MS Analysis: Used as a stable isotope-labeled internal standard for the precise quantification of its non-deuterated counterpart in biological matrices.
• Drug Metabolism Studies (DMPK): Enables tracking and identification of drug metabolites in in vitro and in vivo studies to understand metabolic pathways.
• Pharmacokinetic (PK) Research: Facilitates accurate measurement of drug concentration over time in plasma and tissues, critical for dose determination.
• Bioanalytical Method Development: Serves as a critical reference material for developing and validating robust, sensitive, and specific analytical methods.
• Impurity Profiling and Characterization: Aids in the identification and quantification of process-related impurities and degradation products in API manufacturing.
• Isotope Dilution Mass Spectrometry (IDMS): Employed in high-accuracy quantitative analysis to correct for sample preparation and instrument variability.

Basic Information

Product Name	3,3,3-Trideuterio-2-Hydroxy-n-[4-Nitro-3-(Trifluoromethyl)Phenyl]-2-(Trideuteriomethyl)Propanamide
CAS No.	223134-73-4
Molecular Formula	C12H7D6F3N2O4
Molecular Weight	324.25 g/mol (for C12H7D6F3N2O4)
Synonyms	2-Hydroxy-N-[4-nitro-3-(trifluoromethyl)phenyl]-2-(trideuteriomethyl)-3,3,3-trideuteriopropanamide; Deuterated Bicalutamide Impurity/Intermediate; [D6]-2-Hydroxy-2-methyl-N-[4-nitro-3-(trifluoromethyl)phenyl]propanamide; 2-Hydroxy-2-methyl-N-[4-nitro-3-(trifluoromethyl)phenyl]propanamide-d6; Bicalutamide-d6 (related intermediate); 3,3,3-Trideuterio-2-hydroxy-2-(trideuteriomethyl)-N-[4-nitro-3-(trifluoromethyl)phenyl]propanamide; (RS)-2-Hydroxy-2-methyl-3,3,3-trideuterio-N-[4-nitro-3-(trifluoromethyl)phenyl]propanamide-d3; 4'-Nitro-3'-(trifluoromethyl)isobutyranilide-d6
EINECS	Contact for details

Quality Control

Our deuterated compounds are manufactured under strict quality control protocols to ensure the highest isotopic and chemical purity for research applications. Each batch is characterized using advanced analytical techniques including NMR (¹H, ¹³C, ²H), High-Resolution Mass Spectrometry (HRMS), and HPLC. Certificates of Analysis (COA) are provided, detailing assay, isotopic enrichment (D-content), and impurity profiles. We adhere to relevant guidelines for the production of stable isotope-labeled materials used in regulated bioanalysis.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. For long-term storage, consider storing at 2-8°C. Keep the container tightly sealed to prevent moisture absorption and potential isotopic exchange.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (²H NMR/MS)	≥ 98 atom % D
Chemical Purity (NMR)	Conforms
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.