Description

2-Hydroxy-17Beta-Estradiol-1,4,16,16,17-D5 is a deuterium-labeled analog of the endogenous estrogen metabolite 2-hydroxyestradiol, serving as a critical internal standard for precise analytical quantification. This high-purity compound is essential for ensuring accuracy and reliability in sensitive mass spectrometry-based assays, eliminating variability and enabling definitive identification. It is primarily required by pharmaceutical R&D laboratories, clinical research organizations, and diagnostic manufacturers developing and validating assays for hormone metabolism studies, pharmacokinetics, and biomarker discovery.

Application

• Internal Standard for LC-MS/MS: Primary use as a stable isotope-labeled internal standard for the quantitative bioanalysis of 2-hydroxyestradiol in biological matrices (plasma, serum, urine).
• Metabolite Research: Tracing and quantifying catechol estrogen metabolites in studies of estrogen metabolism pathways and their role in endocrinology and oncology.
• Method Development & Validation: Critical component in the development, calibration, and validation of high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) methods to meet GLP and CLIA standards.
• Clinical Diagnostics: Used in the manufacture of in-vitro diagnostic (IVD) kits and reagents for hormonal profiling and endocrine disorder testing.
• Drug Metabolism & Pharmacokinetics (DMPK): Employed in ADME studies to understand the disposition and biotransformation of estrogenic compounds and related therapeutics.
• Quality Control Reference: Serves as a certified reference material (CRM) for quality assurance and control in analytical laboratories.

Basic Information

Product Name	2-Hydroxy-17Beta-Estradiol-1,4,16,16,17-D5
CAS No.	221093-33-0
Molecular Formula	C18H19D5O3
Molecular Weight	291.40 g/mol (for the deuterated form)
Synonyms	2-Hydroxyestradiol-d5; 2-OHE2-d5; 1,3,5(10)-Estratrien-2,3,17β-triol-1,4,16,16,17-d5; 2,3,17β-Trihydroxyestra-1,3,5(10)-triene-1,4,16,16,17-d5; Estra-1,3,5(10)-triene-2,3,17β-triol-1,4,16,16,17-d5; Deuterated 2-Hydroxyestradiol; [1,4,16,16,17-2H5]-2-Hydroxyestradiol
EINECS	Contact for details

Quality Control

Every batch of 2-Hydroxy-17Beta-Estradiol-1,4,16,16,17-D5 is manufactured and analyzed under strict quality systems. We guarantee high chemical and isotopic purity suitable for use as a critical reference standard. Our products undergo rigorous quality testing, including NMR and mass spectrometry, to ensure compliance with industry standards. Comprehensive Certificates of Analysis (COA) detailing purity, isotopic enrichment, and analytical methods are provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere for long-term stability. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC-MS)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 98 atom % D
Chemical Purity (by NMR)	≥ 97.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.