Description

Bis[1-(2-Nitrophenyl)Ethyl] N,n-Diisopropylphosphoramidite is a specialized phosphoramidite monomer, a critical building block in the synthesis of oligonucleotides and other complex organic molecules. Its primary value lies in its role as a key reagent in solid-phase phosphoramidite chemistry, enabling the precise and automated construction of DNA and RNA sequences. This high-purity intermediate is essential for research and production in the pharmaceutical, biotechnology, and diagnostic industries, particularly for applications in antisense therapy, siRNA, and genetic analysis.

Application

• Oligonucleotide Synthesis: A fundamental phosphoramidite monomer for the automated solid-phase synthesis of DNA and RNA strands.
• Antisense & siRNA Therapeutics: Used in the production of therapeutic oligonucleotides designed to target specific mRNA sequences.
• Primer & Probe Manufacturing: Essential for creating high-fidelity primers and labeled probes used in PCR, qPCR, and genomic sequencing.
• Research & Development: A key reagent in academic and industrial labs for exploring novel nucleic acid analogs and modified oligonucleotides.
• Diagnostic Kits: Incorporated into the manufacture of oligonucleotide components for various molecular diagnostic assays and kits.
• Synthesis of Modified Nucleotides: Serves as a precursor for creating nucleotides with specific backbone or sugar modifications.

Basic Information

Product Name	Bis[1-(2-Nitrophenyl)Ethyl] N,n-Diisopropylphosphoramidite
CAS No.	207516-14-1
Molecular Formula	C22H30N3O4P
Molecular Weight	431.47 g/mol
Synonyms	1-(2-Nitrophenyl)ethyl diisopropylphosphoramidite dimer; N,N-Diisopropyl-bis[1-(2-nitrophenyl)ethyl]phosphoramidite; Bis(1-(2-nitrophenyl)ethyl) N,N-diisopropylphosphoramidite; Phosphoramidite monomer CE; 2-Nitrophenyl ethyl phosphoramidite dimer; DNA synthesis reagent CE phosphoramidite
EINECS	Contact for details

Quality Control

Our Bis[1-(2-Nitrophenyl)Ethyl] N,n-Diisopropylphosphoramidite is manufactured under strict quality systems to ensure it meets the exacting standards required for oligonucleotide synthesis. Each batch is subjected to rigorous analytical testing, including HPLC for purity and NMR for structural confirmation, to guarantee consistency and performance. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and moisture content is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store under an inert atmosphere (e.g., argon or nitrogen) in a cool, dry place at 2-8°C to maintain stability. This compound is hygroscopic (moisture-sensitive) and easily oxidized; prolonged exposure to air, moisture, or elevated temperatures should be avoided.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in anhydrous acetonitrile, THF, DCM

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.