Description

Ethylenediaminetetraacetic-D12 Acid is a deuterated analog of the classic chelating agent EDTA, where twelve hydrogen atoms are replaced by deuterium. This isotopic labeling is critical for applications requiring high chemical stability and minimal spectral interference in analytical techniques. It is essential for researchers and manufacturers in the pharmaceutical, analytical chemistry, and advanced materials sectors who require precise molecular tools for method development and quality control. The compound serves as a key standard and reagent in studies utilizing nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry.

Application

• Internal Standard for Quantitative Analysis: Used as a stable, isotopically labeled internal standard in Liquid Chromatography-Mass Spectrometry (LC-MS) and Gas Chromatography-Mass Spectrometry (GC-MS) for the accurate quantification of EDTA and related compounds.
• NMR Spectroscopy Studies: Employed as a deuterated tracer or shift reagent in Nuclear Magnetic Resonance (NMR) to investigate molecular structures, dynamics, and metal-ligand interactions without proton signal interference.
• Metabolomics and Proteomics Research: Facilitates precise tracking and quantification in complex biological matrices for advanced life science research and biomarker discovery.
• Pharmaceutical Method Development: Supports the development and validation of robust analytical methods for drug substances and products where EDTA is a component or impurity.
• Environmental Analysis: Aids in the sensitive detection and measurement of chelating agents in environmental samples.
• Chemical Kinetics and Mechanism Studies: Used to elucidate reaction pathways and mechanisms involving metal chelation through isotopic labeling techniques.

Basic Information

Product Name	Ethylenediaminetetraacetic-D12 Acid
CAS No.	203806-08-0
Molecular Formula	C10D12H4N2O8
Molecular Weight	300.26 g/mol (theoretical)
Synonyms	EDTA-D12; Deuterated EDTA; (Carboxy-D2-methyl) D8-ethylenedinitrilotetraacetic Acid; Ethylenedinitrilotetraacetic-D12 Acid; Edetic Acid-D12; Perdeuterated EDTA; Tetrasodium EDTA-D12 (for salt form reference); N,N'-1,2-ethanediylbis[N-(carboxymethyl)-D2-glycine]-D8
EINECS	Contact for details

Quality Control

Every batch of Ethylenediaminetetraacetic-D12 Acid is produced and tested under strict quality management protocols. We ensure high isotopic purity and chemical purity through advanced analytical techniques including NMR, MS, and HPLC. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing parameters such as isotopic enrichment, chemical purity, and residual solvent content to meet the stringent requirements of research and analytical applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated area at a controlled room temperature (15-25°C). Due to its hygroscopic nature, the container must be kept tightly sealed after use to minimize exposure to atmospheric moisture. For long-term storage, consider the use of a desiccator.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (NMR)	Conforms to structure
Isotopic Purity (D-content)	≥ 98 atom % D
Chemical Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.