Description

(±)-1,4-Dithiothreitol-D10 CAS NO 203633-21-0 is a deuterated isotopologue of the classic reducing agent 1,4-dithiothreitol (DTT), where ten hydrogen atoms are replaced by deuterium. This deuteration makes it an essential internal standard for quantitative mass spectrometry, enabling precise and accurate measurement of endogenous DTT levels in complex biological matrices. It is primarily needed by analytical laboratories and research institutions in the pharmaceutical, biotechnology, and metabolomics sectors for method development, validation, and high-fidelity bioanalytical studies.

Application

• Internal Standard for LC-MS/MS: Serves as a critical, non-interfering internal standard for the precise quantification of native DTT in biological samples (e.g., plasma, serum, cell lysates) using liquid chromatography-tandem mass spectrometry.
• Deuterated Tracer Studies: Used in metabolic pathway analysis and pharmacokinetic studies to trace the fate of thiol-containing compounds without isotopic interference.
• Proteomics & Protein Chemistry: Employed in disulfide bond reduction experiments where isotopic labeling is required for downstream mass spectrometric analysis of peptides and proteins.
• Method Development & Validation: Essential for developing and validating robust, GLP/GMP-compliant analytical methods that require isotope-dilution techniques for maximum accuracy.
• Quality Control Reference Material: Acts as a certified reference material in quality control laboratories to ensure the accuracy of analytical instruments and procedures.
• Stable Isotope Labeling: Facilitates research in chemical mechanisms and reaction kinetics where deuterium labeling provides distinct spectroscopic signatures.

Basic Information

Product Name	(±)-1,4-Dithiothreitol-D10
CAS No.	203633-21-0
Molecular Formula	C4D10O2S2
Molecular Weight	162.33 g/mol
Synonyms	DTT-d10; Deuterated DTT; (R*,S*)-1,4-Dimercapto-2,3-butanediol-d10; meso-1,4-Dimercapto-2,3-butanediol-d10; Cleland's Reagent-d10; (±)-threo-1,4-Dimercapto-2,3-butanediol-d10; 2,3-Dihydroxy-1,4-butanedithiol-d10; 1,4-Dithio-DL-threitol-d10
EINECS	Contact for details

Quality Control

Our (±)-1,4-Dithiothreitol-D10 is manufactured under strict quality systems to ensure high isotopic purity and chemical integrity, critical for its role as an analytical standard. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing parameters such as isotopic purity (D-enrichment), chemical purity (by HPLC), and identity confirmation. We support GMP/GLP-compliant research and are committed to providing materials that meet the exacting requirements of modern analytical laboratories.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C under an inert atmosphere (e.g., nitrogen or argon) due to its properties of being easily oxidized and hygroscopic. Allow the container to reach room temperature before opening to minimize moisture ingress.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D-enrichment)	≥ 98 atom % D
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.