Description

L-Allo-Isoleucine (D10) is a stable isotope-labeled, non-proteinogenic amino acid, specifically the allo-isomer of isoleucine. This high-purity compound is essential for advanced research and development where precise metabolic tracing and structural analysis are required. It is primarily utilized by pharmaceutical researchers, analytical laboratories, and manufacturers of isotopically labeled standards for applications in mass spectrometry, NMR spectroscopy, and metabolic studies.

Application

• Internal Standard for Mass Spectrometry (MS): Serves as a critical quantitative reference standard in LC-MS and GC-MS analysis for accurate measurement of isoleucine and related metabolites in complex biological matrices.
• Metabolic Pathway Tracing: Used in in vivo and in vitro studies to track and elucidate biochemical pathways involving branched-chain amino acids (BCAAs) using stable isotope labeling techniques.
• NMR Spectroscopy Studies: Provides a deuterium-labeled probe for nuclear magnetic resonance (NMR) spectroscopy, enabling detailed structural and dynamic analysis of proteins and peptides.
• Pharmaceutical R&D: Employed in the research and development of new therapeutic agents, particularly in pharmacokinetic (PK) and pharmacodynamic (PD) studies requiring labeled tracers.
• Manufacturing of Labeled Standards: Acts as a key building block for the synthesis of more complex, custom deuterium-labeled compounds and certified reference materials (CRMs).
• Nutritional and Biomedical Research: Facilitates studies on amino acid metabolism, protein synthesis, and disorders related to BCAA catabolism.

Basic Information

Product Name	L-Allo-Isoleucine (D10)
CAS No.	202529-06-4
Molecular Formula	C6H3D10NO2
Molecular Weight	136.23 g/mol (for unlabeled C6H13NO2; Contact for exact labeled MW)
Synonyms	(2S,3S)-2-Amino-3-methylpentanoic acid-d10; L-Alloisoleucine-d10; (2S,3S)-2-Azaniumyl-3-methylpentanoate-d10; allo-Ile-d10; Deuterated L-allo-isoleucine; L-α-Amino-β-methylvaleric acid-d10; Stable Isotope Labeled L-Allo-Isoleucine
EINECS	Contact for details

Quality Control

Our L-Allo-Isoleucine (D10) is manufactured under strict quality systems to ensure exceptional isotopic and chemical purity, critical for research integrity. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing parameters such as isotopic enrichment (atom % D), chemical purity by HPLC, chiral purity, and residual solvent content. We adhere to relevant guidelines for the production of stable isotope-labeled compounds to guarantee batch-to-batch consistency and reliability for your most demanding applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation or storage under an inert atmosphere to maintain stability and isotopic integrity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Isotopic Enrichment	≥ 98 atom % D
Chemical Purity (HPLC)	≥ 98.0%
Chiral Purity (HPLC)	≥ 99.0% (L-allo form)
Specific Rotation	Contact for details
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.