Description

Isoleucine-D10 (Mixture Of Diastereomers) is a stable isotope-labeled analog of the essential amino acid isoleucine, where ten hydrogen atoms are replaced by deuterium. This compound is a critical tool for precise quantitative analysis and metabolic pathway tracing in advanced research. It is primarily utilized by scientists and quality control professionals in the pharmaceutical, biotechnology, and nutritional science industries for applications requiring high specificity and accuracy.

Application

• Internal Standard for Mass Spectrometry (MS): Serves as a crucial reference compound for the accurate quantification of endogenous isoleucine in complex biological matrices like plasma, urine, and tissue samples.
• Metabolic Research & Tracer Studies: Used to investigate amino acid metabolism, protein turnover, and metabolic fluxes in both in vitro and in vivo systems, providing insights into disease mechanisms and nutritional status.
• Pharmaceutical Development & Bioanalysis: Essential in pharmacokinetic (PK) and pharmacodynamic (PD) studies to measure drug metabolism and the dynamics of amino acid-based therapeutics.
• Clinical Diagnostics: Enables precise measurement of isoleucine levels for the diagnosis and monitoring of metabolic disorders such as Maple Syrup Urine Disease (MSUD).
• Nutritional Science & Supplement Analysis: Employed in the analysis of protein hydrolysates, infant formula, and dietary supplements to ensure accurate amino acid profiling and label claim verification.
• Proteomics & Biomarker Discovery: Facilitates relative and absolute quantification in proteomic workflows to identify and validate protein biomarkers.

Basic Information

Product Name	Isoleucine-D10 (Mixture Of Diastereomers)
CAS No.	29909-02-2
Molecular Formula	C6D10H3NO2
Molecular Weight	137.20 g/mol (for unlabeled base: 131.17 g/mol)
Synonyms	(2S,3S)-2-Amino-3-methylpentanoic-3,4,4,5,5,5-d6 acid-d4; L-Isoleucine-d10; (2S,3S)-2-Amino-3-methylpentanoic-d10 acid; Deuterated Isoleucine; ILE-d10; Perdeuterated Isoleucine; Iso-leucine-d10; 2-Amino-3-methylvaleric acid-d10
EINECS	Contact for details

Quality Control

Our Isoleucine-D10 is manufactured under strict quality systems to ensure the high isotopic purity and chemical integrity required for sensitive analytical applications. Each batch is characterized by advanced techniques including NMR, Mass Spectrometry, and HPLC to confirm isotopic enrichment, chemical purity, and diastereomeric composition. A comprehensive Certificate of Analysis (COA) detailing these specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a cool, dry place. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (MS)	≥ 98.0 atom % D
Chemical Purity	≥ 95.0%
Diastereomeric Ratio (Isoleucine/Allo-isoleucine)	Typically ~95:5
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.