Description

Thymolphthalein Monophosphate Disodium Salt is a chromogenic substrate used in enzymatic assays. This compound is critical for the detection and quantification of alkaline phosphatase activity, a key biomarker in clinical diagnostics and biochemical research. It is primarily utilized by manufacturers of diagnostic kits, research laboratories in life sciences, and quality control departments in the pharmaceutical industry.

Application

• Clinical Diagnostic Kits: As a key chromogenic substrate in diagnostic assays for measuring alkaline phosphatase (ALP) levels in serum, a vital marker for liver and bone disorders.
• Biochemical Research: For studying enzyme kinetics, inhibition, and activity of alkaline phosphatase in various in vitro experimental models.
• Pharmaceutical QC: Used in quality control testing of biopharmaceuticals where alkaline phosphatase is a process-related impurity or a critical quality attribute.
• Immunoassays (ELISA): Employed as a substrate in enzyme-linked immunosorbent assays for colorimetric detection.
• Histochemistry & Cytochemistry: For the localization of alkaline phosphatase activity in tissue sections and cell cultures.
• Molecular Biology: In nucleic acid detection systems that utilize alkaline phosphatase-conjugated probes.
• Water & Environmental Testing: Potential use in monitoring microbial contamination where alkaline phosphatase serves as an indicator enzyme.
• Food Safety Analysis: For developing assays to detect bacterial contamination (e.g., in dairy products) based on enzymatic activity.

Basic Information

Product Name	Thymolphthalein Monophosphate Disodium Salt
CAS No.	28749-63-5
Molecular Formula	C31H28O10PNa2
Molecular Weight	636.49 g/mol
Synonyms	Thymolphthalein Monophosphate, Disodium Salt; Thymolphthalein Phosphate, Disodium Salt; 5',5''-Diisopropyl-2',2''-dimethylphenolphthalein Phosphate Disodium Salt; Thymolphthalein Diphosphate Disodium Salt (common misnomer); TMP Disodium Salt; ALP Substrate; Diagnostic Substrate TMP; 3,3-Bis[4-hydroxy-2-methyl-5-(propan-2-yl)phenyl]-1(3H)-isobenzofuranone Monophosphate Disodium Salt
EINECS	Contact for details

Quality Control

Our Thymolphthalein Monophosphate Disodium Salt is manufactured under a quality management system. Each batch undergoes rigorous analytical testing to ensure high purity, consistent performance, and compliance with relevant standards for diagnostic and research use. Certificates of Analysis (COA) documenting identity, purity (HPLC), and functional performance are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to prevent absorption of atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Clear, colorless solution in water
Functional Test (Enzymatic)	Conforms to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.