Description

Uracil-D4 CAS NO 24897-55-0 is a stable isotope-labeled analog of uracil, where four hydrogen atoms are replaced by deuterium. This compound is essential as an internal standard in quantitative mass spectrometry, ensuring unparalleled accuracy and precision in analytical measurements. It is a critical tool for researchers and quality control laboratories in pharmaceutical development, clinical diagnostics, and metabolic studies who require definitive data for method validation and regulatory compliance.

Application

• Quantitative Mass Spectrometry (LC-MS/MS, GC-MS): Serves as a critical internal standard for the accurate quantification of uracil and related pyrimidine metabolites in complex biological matrices.
• Pharmaceutical R&D and Bioanalysis: Used in pharmacokinetic (PK) and pharmacodynamic (PD) studies to track drug metabolism and ensure reliable assay performance for regulatory submissions.
• Clinical Diagnostics and Metabolomics: Enables precise measurement of uracil levels in plasma or urine for research into metabolic disorders, such as those related to the pyrimidine degradation pathway.
• Stable Isotope Labeling Studies: Employed as a tracer in metabolic flux analysis to investigate nucleotide biosynthesis and turnover in cell culture and in vivo models.
• Quality Control for Nucleotide APIs: Supports the impurity profiling and assay validation of uracil-based active pharmaceutical ingredients (APIs) and finished drug products.
• Forensic and Doping Control Analysis: Provides a reliable reference standard for the confirmatory analysis of endogenous compounds in forensic toxicology and anti-doping programs.

Basic Information

Product Name	Uracil-D4
CAS No.	24897-55-0
Molecular Formula	C4D4N2O2
Molecular Weight	116.10 g/mol
Synonyms	2,4-Dioxopyrimidine-d4; 2,4(1H,3H)-Pyrimidinedione-d4; Deuterated Uracil; Uracil-d4; [2,4,5,6-2H4]Uracil; Perdeuterouracil (D4); NSC 27420-d4
EINECS	Contact for details

Quality Control

Every batch of Uracil-D4 is manufactured and tested under strict quality management systems. We provide comprehensive analytical data to ensure the product meets the high standards required for sensitive analytical applications. A detailed Certificate of Analysis (COA) is supplied with each shipment, reporting key parameters including isotopic purity (D-enrichment), chemical purity by HPLC, and confirmation of structure by spectroscopic methods.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to prevent absorption of atmospheric moisture, which could affect stability and analytical performance.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D-enrichment)	≥ 98 atom % D
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.