Description

Uracil-5-D1 is a deuterium-labeled derivative of the fundamental pyrimidine nucleobase uracil, specifically deuterated at the 5-position. This isotopic labeling makes it an indispensable internal standard and tracer in advanced analytical and metabolic research, ensuring precise quantification and reliable data integrity. It is primarily utilized by research scientists and process developers in the pharmaceutical, biotechnology, and agrochemical sectors for applications in mass spectrometry, NMR spectroscopy, and drug metabolism studies.

Application

• Internal Standard for LC-MS/MS: Critical for the accurate quantification of uracil and related compounds in complex biological matrices like plasma, urine, and tissue homogenates.
• Metabolic Pathway Tracing: Used in pharmacokinetic (PK) and absorption, distribution, metabolism, and excretion (ADME) studies to track the fate of nucleobase analogs in vivo.
• NMR Spectroscopy: Serves as a valuable probe in nuclear magnetic resonance studies to elucidate molecular structure, dynamics, and interaction mechanisms without signal interference from protons.
• Stable Isotope Labeling: Employed in the synthesis of deuterated active pharmaceutical ingredients (APIs) and other labeled biomolecules for research and development.
• Biochemical Research: A key reagent in enzymatic studies, nucleotide metabolism research, and investigations into nucleic acid chemistry and repair mechanisms.
• Quality Control Reference: Acts as a certified reference material (CRM) for method validation and quality assurance in analytical laboratories.

Basic Information

Product Name	Uracil-5-D1
CAS No.	24897-50-5
Molecular Formula	C₄H₃D₁N₂O₂
Molecular Weight	113.09 g/mol
Synonyms	5-Deuteriouracil; [5-²H]Uracil; Uracil-d1; 2,4-Dihydroxypyrimidine-5-d1; 5-Deutero-2,4(1H,3H)-pyrimidinedione; Uracil-5-d; 5-D-Uracil; 2,4(1H,3H)-Pyrimidinedione-5-d
EINECS	Contact for details

Quality Control

Our Uracil-5-D1 is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for chemical purity and isotopic enrichment analysis (e.g., NMR, MS) to confirm deuterium incorporation. We provide comprehensive Certificates of Analysis (COA) detailing all specifications, including assay, isotopic purity, and identification. Our quality commitment ensures the material meets the high standards required for sensitive analytical and research applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This compound is hygroscopic (moisture-sensitive); therefore, the container must be kept tightly sealed in a dry environment to prevent degradation. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Isotopic Purity (Deuterium Content)	≥ 98 atom % D
Chemical Purity (by HPLC)	≥ 99.0%
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.