Description

Uracil-1,3-D2 is a deuterium-labeled isotopologue of the fundamental pyrimidine nucleobase uracil, specifically deuterated at the 1 and 3 nitrogen positions. This stable isotope-labeled compound is critical for advanced analytical and research applications where precise molecular tracking and quantification are required. It is primarily utilized by researchers and manufacturers in the pharmaceutical, agrochemical, and analytical chemistry sectors for use as an internal standard in mass spectrometry, metabolic pathway studies, and the synthesis of labeled nucleosides and nucleotides.

Application

• Internal Standard for Quantitative Analysis: Serves as a high-purity internal standard in Liquid Chromatography-Mass Spectrometry (LC-MS) and Gas Chromatography-Mass Spectrometry (GC-MS) for the accurate quantification of uracil and related compounds in complex biological matrices.
• Metabolic Research & Tracer Studies: Used in pharmacokinetic, metabolomic, and drug metabolism studies to trace biochemical pathways and understand the fate of nucleobase analogs in vivo.
• Synthesis of Deuterated Biomolecules: A key building block for the synthesis of more complex deuterium-labeled nucleosides, nucleotides, and oligonucleotides for NMR spectroscopy and other structural biology techniques.
• Pharmaceutical Development: Employed in the research and development of novel antiviral and anticancer agents that target nucleic acid metabolism, aiding in mechanism-of-action studies.
• Environmental & Agricultural Science: Can be used as a tracer in studies examining the environmental fate and metabolic breakdown of pyrimidine-based agrochemicals.
• Reference Material: Provides a certified reference material for analytical method development, validation, and quality control in regulatory-compliant laboratories.

Basic Information

Product Name	Uracil-1,3-D2
CAS No.	20666-60-8
Molecular Formula	C₄H₂D₂N₂O₂
Molecular Weight	114.10 g/mol
Synonyms	1,3-Dideuteriouracil; [1,3-²H₂]Uracil; Uracil-d2; 2,4(1H,3H)-Pyrimidinedione-1,3-d2; Deuterated Uracil; Uracil (1,3-D2); NSC 403042; 2,4-Dihydroxypyrimidine-1,3-d2
EINECS	Contact for details

Quality Control

Our Uracil-1,3-D2 is manufactured under controlled conditions to ensure high chemical and isotopic purity, meeting the stringent requirements of research and analytical applications. Each batch undergoes rigorous analysis, including NMR spectroscopy to confirm deuteration level and position, and HPLC for chemical purity assessment. A comprehensive Certificate of Analysis (COA) detailing isotopic enrichment (typically >98 atom % D), chemical purity, and analytical results is provided with every shipment to guarantee traceability and consistency for your critical work.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Isotopic Purity (Atom % D)	≥ 98% (by ¹H-NMR)
Chemical Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.