Description

2,3-Diphenyl-5-Cyanotetrazolium Chloride is a specialized tetrazolium salt widely recognized for its role as a redox indicator and chromogenic substrate in biochemical assays. This compound is critical for researchers and industrial laboratories requiring precise detection of cellular metabolic activity and enzymatic function. It is primarily utilized in the life sciences, pharmaceutical development, and diagnostic reagent manufacturing sectors.

Application

• Cell Viability and Proliferation Assays (e.g., MTT Assay Alternative): Used as a substrate for dehydrogenase enzymes in mitochondria to measure metabolic activity.
• Biochemical Research: Acts as an electron acceptor in studies of enzyme kinetics, particularly for reductases and dehydrogenases.
• Diagnostic Reagent Formulation: Incorporated into kits for clinical diagnostics to detect bacterial contamination or specific enzymatic pathways.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to evaluate the cytotoxicity of new drug candidates.
• Histochemistry: Used as a staining agent to localize enzyme activity in tissue samples.
• Material Science Research: Investigated for its properties in the development of novel electrochromic or photochromic materials.

Basic Information

Product Name	2,3-Diphenyl-5-Cyanotetrazolium Chloride
CAS No.	2118-44-7
Molecular Formula	C20H13ClN6
Molecular Weight	372.81 g/mol
Synonyms	DPCTC; 5-Cyano-2,3-diphenyltetrazolium Chloride; Tetrazolium Chloride, 2,3-Diphenyl-5-cyano-; 2,3-Diphenyl-5-tetrazoliumcyanide Chloride; Cyanotetrazolium Blue Chloride; CTC; 2,3-Diphenyl-5-cyanotetrazolium chloride; 2,3-Diphenyltetrazolium-5-cyanide Chloride
EINECS	Contact for details

Quality Control

Our 2,3-Diphenyl-5-Cyanotetrazolium Chloride is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure batch-to-batch consistency and high purity suitable for research and industrial applications. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing key parameters such as assay, impurity profile, and spectral identification. Our quality system is designed to meet the stringent requirements of GMP and ISO standards where applicable.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture or light may affect stability and performance. Ensure the container is kept sealed when not in use.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Loss on Drying	≤ 2.0%
Residue on Ignition	≤ 0.5%
Heavy Metals (as Pb)	≤ 20 ppm
Solubility	Soluble in DMSO, methanol; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.