Description

Phenolphthalein Diphosphate is a high-purity biochemical reagent primarily used as a chromogenic substrate for enzyme detection. This compound is critical for analytical and diagnostic applications where precise and sensitive colorimetric detection is required. It is an essential material for research laboratories, diagnostic kit manufacturers, and quality control departments in the pharmaceutical and biotechnology sectors.

Application

• Enzyme-Linked Immunosorbent Assay (ELISA): Used as a substrate for alkaline phosphatase (ALP) in diagnostic assays.
• Clinical Diagnostics: A key component in kits for detecting specific biomarkers and enzymatic activity in biological samples.
• Biochemical Research: Employed in enzymatic studies and as a tool for visualizing phosphatase activity in gel electrophoresis and histochemistry.
• Pharmaceutical Quality Control: Utilized in testing procedures to ensure the purity and activity of enzyme-based pharmaceuticals.
• Food Safety Testing: Applied in assays to detect bacterial contamination (e.g., E. coli) via associated phosphatase activity.
• Molecular Biology: Serves as a substrate in colorimetric detection systems for nucleic acid hybridization and blotting techniques.

Basic Information

Product Name	Phenolphthalein Diphosphate
CAS No.	2090-82-6
Molecular Formula	C20H16O10P2
Molecular Weight	478.28 g/mol
Synonyms	Phenolphthalein Diphosphoric Acid Ester; Phenolphthalein Bis(phosphate); 3,3-Bis(4-hydroxyphenyl)-1(3H)-isobenzofuranone Diphosphate; Phenolphthalein Diphosphoric Acid; ALP Substrate; Phosphatase Substrate; Diagnostic Reagent 2090-82-6
EINECS	218-229-4

Quality Control

Our Phenolphthalein Diphosphate is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the stringent requirements for diagnostic and research applications. Certificates of Analysis (COA) detailing all specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, buffer solutions
Heavy Metals	≤ 20 ppm
Residual Solvents	Meets ICH guidelines
Enzymatic Activity	Suitable for use as ALP substrate

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.