Description

D-Proline-2,5,5-D3 is a deuterium-labeled isotopologue of the non-proteinogenic amino acid D-proline, specifically labeled at the 2, 5, and 5 positions. This compound is of significant value as a stable isotope-labeled internal standard in quantitative mass spectrometry, ensuring precise and accurate analytical measurements. It is primarily needed by pharmaceutical research and development laboratories, contract research organizations (CROs), and academic institutions engaged in advanced metabolomic, proteomic, and pharmacokinetic studies.

Application

• Mass Spectrometry Internal Standard: Serves as a critical reference compound for the accurate quantification of proline and related metabolites in complex biological matrices via LC-MS/MS and GC-MS.
• Drug Metabolism and Pharmacokinetics (DMPK): Used in ADME (Absorption, Distribution, Metabolism, Excretion) studies to track the fate of drug candidates containing proline moieties.
• Biomedical Research: Enables precise tracking in studies of collagen synthesis, cellular stress responses, and neurological pathways where proline plays a key role.
• Metabolomics and Biomarker Discovery: Facilitates the absolute quantification of proline levels in biofluids for disease state research and diagnostic development.
• Isotope Tracing Experiments: Acts as a tracer in metabolic flux analysis to elucidate biochemical pathways in cell cultures and model organisms.
• Quality Control for Peptide Synthesis: Employed in the analysis and quality assurance of synthetic peptides and oligonucleotides where D-proline is incorporated.

Basic Information

Item	Details
Product Name	D-Proline-2,5,5-D3
CAS No.	1994265-09-6
Molecular Formula	C5H4D3NO2
Molecular Weight	118.13 g/mol
Synonyms	(2R)-Pyrrolidine-2-carboxylic-2,5,5-d3 acid; (R)-(-)-Proline-2,5,5-d3; D(-)-Proline-2,5,5-d3; (R)-Proline-2,5,5-d3; Deuterated D-Proline; D-Proline-d3; 2,5,5-Trideutero-D-proline; (R)-2-Carboxypyrrolidine-2,5,5-d3
EINECS	Contact for details

Quality Control

Our D-Proline-2,5,5-D3 is manufactured under strict quality systems to ensure the high chemical and isotopic purity required for sensitive analytical applications. Each batch is characterized by advanced spectroscopic and chromatographic techniques, including NMR, HPLC, and mass spectrometry, to confirm identity, chemical purity, and deuterium enrichment. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. This material is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D-enrichment)	≥ 98 atom % D
Chemical Purity (NMR)	Conforms
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.