Description

13C6-3-Nitrophenylhydrazine (Hydrochloride) is a stable isotope-labeled derivative of 3-nitrophenylhydrazine, a key reagent used for the derivatization and analysis of carbonyl compounds. This high-purity labeled compound is essential for ensuring accuracy and reliability in quantitative mass spectrometry, where it serves as an internal standard to correct for analyte loss and instrument variability. It is primarily required by analytical laboratories and research institutions in the pharmaceutical, environmental, and metabolomics fields for precise LC-MS/MS quantification of aldehydes and ketones.

Application

• Used as an internal standard in quantitative LC-MS/MS analysis of carbonyl-containing metabolites in biological samples.
• Critical for the derivatization and accurate measurement of short-chain aldehydes (e.g., formaldehyde, acetaldehyde) in environmental and occupational health studies.
• Employed in pharmaceutical impurity profiling and stability testing to quantify degradation products that contain aldehyde or ketone functional groups.
• Facilitates high-sensitivity detection in clinical diagnostics for disease biomarkers that are carbonyl compounds.
• Applied in food and flavor chemistry research for the precise analysis of aroma-active carbonyl molecules.
• Supports isotope dilution mass spectrometry (IDMS) methodologies, which are considered the gold standard for definitive quantification.

Basic Information

Product Name	13C6-3-Nitrophenylhydrazine (Hydrochloride)
CAS No.	1977535-33-3
Molecular Formula	C613C6H9N3O2·HCl
Molecular Weight	Contact for details
Synonyms	3-Nitrophenyl-13C6-hydrazine hydrochloride; (3-Nitrophenyl)hydrazine-13C6 hydrochloride; 13C6-Labeled 3-NPH·HCl; 13C6-3-NPH·HCl; 3-NPH-13C6 hydrochloride; Isotopically labeled DNPH derivative; Carbon-13 labeled DNPH analog
EINECS	Contact for details

Quality Control

Our 13C6-3-Nitrophenylhydrazine (Hydrochloride) is manufactured under strict quality systems to ensure the high isotopic purity and chemical integrity required for trace analysis. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing isotopic enrichment (typically >99 atom% 13C), chemical purity, and confirming the absence of interfering unlabeled species. We adhere to cGMP principles for the production of reference standards used in regulated laboratories.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a cool, dry place. This material is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Isotopic Purity (13C)	≥99 atom%
Chemical Purity (HPLC)	≥98.0%
Unlabeled Impurity (HPLC)	≤0.5%
Water Content (KF)	≤1.0%
Solubility	Soluble in methanol, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.