Description

Thiol-Dpeg4-T-Boc-n-Amido is a specialized heterobifunctional polyethylene glycol (PEG) linker featuring a thiol group and a tert-butyloxycarbonyl (Boc)-protected amine. This compound is engineered to facilitate the controlled conjugation of biomolecules, offering precise spacing and solubility enhancement. It is a critical reagent for researchers and manufacturers in the fields of bioconjugation, proteomics, and advanced drug delivery system development, particularly for antibody-drug conjugates (ADCs) and peptide therapeutics.

Application

• Bioconjugation and Crosslinking: Used as a heterobifunctional crosslinker to connect thiol-containing molecules (e.g., cysteine residues in proteins/peptides) with amine-containing molecules after Boc deprotection.
• Antibody-Drug Conjugate (ADC) Development: Serves as a key component in the synthesis of linker-payload constructs, providing a hydrophilic PEG spacer to improve solubility and pharmacokinetics of the final conjugate.
• Peptide and Protein Modification: Enables site-specific PEGylation (PEG modification) to enhance the stability, half-life, and bioavailability of therapeutic peptides and proteins.
• Surface Functionalization: Utilized to modify nanoparticles, biosensors, and microarray surfaces with specific functional groups for subsequent biomolecule attachment.
• Solid-Phase Peptide Synthesis (SPPS): Acts as a building block or handle for introducing PEG spacers and protected amine functionalities during peptide chain assembly.
• Diagnostic Reagent Formulation: Employed in the development of diagnostic assays and imaging probes that require stable, specific linkages between different molecular entities.

Basic Information

Product Name	Thiol-Dpeg4-T-Boc-n-Amido
CAS No.	1895922-68-5
Molecular Formula	C22H43NO8S
Molecular Weight	481.65 g/mol
Synonyms	1-Amino-3,6,9,12-tetraoxapentadecan-15-oic acid, 15-[(1,1-dimethylethoxy)carbonyl]amino]-13-thio-; Boc-NH-PEG4-SH; Boc-Amino-PEG4-Thiol; tert-Butyl (17-mercapto-3,6,9,12-tetraoxa-16-azaheptadecyl)carbamate; Thiol-PEG4-Boc-amine; Heterobifunctional PEG linker SH-PEG4-NH-Boc; 2,5,8,11-Tetraoxatetradecane-14-thiol, 14-[[(1,1-dimethylethoxy)carbonyl]amino]-
EINECS	Contact for details

Quality Control

Our Thiol-Dpeg4-T-Boc-n-Amido is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity assessment, NMR for structural confirmation, and titration for functional group quantification, to ensure it meets the high standards required for research and pharmaceutical applications. Certificates of Analysis (COA) with detailed specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to prevent oxidation and degradation. Allow the container to reach room temperature in a desiccator before opening to minimize moisture absorption. For long-term storage, keep desiccated at -20°C.

Specification

Item	Specification
Appearance	Colorless to pale yellow viscous liquid or oil
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Assay (Titration)	≥ 95.0%
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.