Description

Necrosulfonamide-D4 CAS NO 1795144-22-7 is a deuterated analog of the selective MLKL inhibitor, Necrosulfonamide, designed for use as an internal standard in quantitative mass spectrometry. This stable isotope-labeled compound is critical for ensuring analytical accuracy and reproducibility in pharmacokinetic and metabolic studies. It is primarily utilized by pharmaceutical researchers and contract research organizations (CROs) engaged in the development of novel therapeutics targeting necroptosis pathways.

Application

• Internal Standard for LC-MS/MS: Serves as a crucial internal standard for the precise quantification of Necrosulfonamide in biological matrices (e.g., plasma, tissue homogenates) using liquid chromatography-tandem mass spectrometry (LC-MS/MS).
• Pharmacokinetic (PK) Studies: Enables accurate tracking of drug absorption, distribution, metabolism, and excretion (ADME) profiles in preclinical and clinical research.
• Metabolite Identification: Aids in the identification and quantification of drug metabolites by providing a stable isotopic reference point in complex analytical samples.
• Biomarker Research: Supports quantitative proteomics and biomarker discovery studies focused on the MLKL protein and necroptosis signaling pathways.
• Drug Development & Validation: Essential for method development, validation, and ensuring Good Laboratory Practice (GLP) compliance in analytical laboratories.
• High-Throughput Screening (HTS): Can be employed in assay development for high-throughput screening of compound libraries targeting regulated cell death mechanisms.

Basic Information

Product Name	Necrosulfonamide-D4
CAS No.	1795144-22-7
Molecular Formula	C₁₇H₁₀D₄N₂O₅S₂
Molecular Weight	~394.46 g/mol
Synonyms	Necrosulfonamide-d4; (E)-N-[4-(Methylsulfonyl)-2-(2-thienylcarbonyl)phenyl]-3-(2-thienyl)-2-propenamide-d4; MLS000576203-d4; MLS-000576203-d4; MLKL Inhibitor-d4; 2-((3-(Thiophene-2-carbonyl)-4-(methylsulfonyl)phenyl)carbamoyl)vinyl)thiophene-3-carboxylate-d4; Deuterated Necrosulfonamide
EINECS	Contact for details

Quality Control

Our Necrosulfonamide-D4 is manufactured under strict quality control protocols to ensure the highest isotopic purity and chemical integrity. Each batch is characterized by advanced analytical techniques including NMR (¹H, ¹³C) and High-Resolution Mass Spectrometry (HRMS) to confirm structure and isotopic enrichment. We guarantee a minimum chemical purity of 95% and isotopic purity ≥98 atom % D. Comprehensive Certificates of Analysis (COA) detailing purity, assay, and solvent content are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to prevent moisture absorption.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (NMR)	Conforms to structure
Identification (HRMS)	Conforms to structure
Purity (HPLC)	≥ 95%
Isotopic Purity	≥ 98 atom % D
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.