Description

Alfacalcidol-D6 is a deuterated analog of the active vitamin D metabolite, Alfacalcidol, specifically labeled with six deuterium atoms. This high-purity stable isotope-labeled compound is essential for use as an internal standard in quantitative mass spectrometry, ensuring unparalleled accuracy and reliability in analytical results. It is critically needed by pharmaceutical R&D laboratories, clinical research organizations, and analytical testing facilities for pharmacokinetic studies, drug metabolism research, and bioanalytical method development.

Application

• Internal Standard for LC-MS/MS Analysis: Primary application for the precise quantification of Alfacalcidol and related metabolites in complex biological matrices like plasma, serum, and tissue homogenates.
• Drug Metabolism and Pharmacokinetics (DMPK) Studies: Enables accurate tracking of drug absorption, distribution, metabolism, and excretion (ADME) in preclinical and clinical research.
• Bioanalytical Method Development and Validation: Serves as a critical reference standard to establish specificity, accuracy, and precision in new assay protocols.
• Clinical Research and Therapeutic Drug Monitoring (TDM): Used in studies investigating vitamin D therapy efficacy and in monitoring patient serum levels of active vitamin D compounds.
• Pharmaceutical Quality Control: Supports impurity profiling and assay verification of Alfacalcidol in active pharmaceutical ingredient (API) and finished dosage form manufacturing.
• Metabolomics and Biomarker Discovery: Facilitates the accurate identification and quantification of vitamin D pathway metabolites in large-scale profiling studies.

Basic Information

Product Name	Alfacalcidol-D6
CAS No.	1641940-94-4
Molecular Formula	C27H38D6O3
Molecular Weight	416.66 g/mol
Synonyms	1α-Hydroxyvitamin D3-D6; 1α-Hydroxycholecalciferol-D6; 1α-OH-D3-D6; (5Z,7E)-9,10-Secocholesta-5,7,10(19)-triene-1α,3β-diol-D6; 1α,25-Dihydroxyvitamin D3 precursor-D6; 1α-Hydroxyergocalciferol-D6 (less common); Alfacalcidol hexadeuterated
EINECS	Contact for details

Quality Control

Every batch of Alfacalcidol-D6 is manufactured and analyzed under strict quality systems to ensure it meets the exacting standards required for analytical and research applications. We provide comprehensive Certificates of Analysis (COA) detailing isotopic purity (e.g., D6 content ≥ 98%), chemical purity (typically ≥ 95% by HPLC), and confirmation of structure. Our quality commitment aligns with the needs of cGMP/GLP-compliant environments, supporting traceability and data integrity.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to air, moisture, or light should be avoided to maintain stability and purity. Allow the sealed vial to equilibrate to room temperature before opening to minimize condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Isotopic Purity (D6)	≥ 98.0 atom % D
Chemical Purity (by HPLC)	Balance to 100%
Solubility	Soluble in ethanol, DMSO; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.