Description

Mal-Peg3-NHS Ester (CAS NO 1537892-36-6) is a heterobifunctional crosslinking reagent featuring a maleimide group and an NHS ester, connected by a triethylene glycol spacer. This compound is essential for creating stable, site-specific conjugates between thiol-containing molecules (like proteins or peptides) and amine-containing targets. It is a critical reagent for researchers and manufacturers in bioconjugation, antibody-drug conjugate (ADC) development, and diagnostic assay formulation, enabling the production of well-defined biomolecular constructs.

Application

• Antibody-Drug Conjugate (ADC) Linker: For the controlled attachment of cytotoxic payloads to monoclonal antibodies via cysteine residues.
• Protein/Peptide Labeling: Site-specific conjugation of fluorescent dyes, enzymes, or other probes to thiol-containing biomolecules for imaging and detection.
• Surface Functionalization: Immobilization of peptides or proteins onto amine-coated biosensors, chips, or nanoparticles to create bioactive surfaces.
• PEGylation Chemistry: Introducing a short, hydrophilic PEG spacer to improve the solubility and pharmacokinetic properties of therapeutic biomolecules.
• Diagnostic Reagent Synthesis: Manufacturing of enzyme-antibody conjugates used in ELISA and other immunoassay kits.
• Biomaterial Crosslinking: Creating crosslinked hydrogels or polymer networks for drug delivery and tissue engineering applications.

Basic Information

Product Name	Mal-Peg3-NHS Ester
CAS No.	1537892-36-6
Molecular Formula	C21H28N2O10
Molecular Weight	468.46 g/mol
Synonyms	Maleimide-PEG3-NHS ester; 3-Maleimidopropionic acid 2,5-dioxopyrrolidin-1-yl ester; Maleimido-PEG3-NHS; NHS-PEG3-Maleimide; 2,5-Dioxopyrrolidin-1-yl 3-((2-(2-(3-maleimidopropanoyloxy)ethoxy)ethoxy)ethyl)carbamate; PEG3 Maleimide NHS Ester; Mal-PEG3-OSu
EINECS	Contact for details

Quality Control

Our Mal-Peg3-NHS Ester is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity for sensitive bioconjugation applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional performance. We adhere to cGMP principles where applicable to support our customers in preclinical and diagnostic development.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to prevent condensation. For long-term stability, store under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.