Description

Secalciferol-D6 is a deuterium-labeled analog of vitamin D2, specifically designed for use as an internal standard in analytical chemistry. This high-purity compound is essential for ensuring the accuracy and reliability of quantitative analyses in complex matrices. It is primarily utilized by pharmaceutical R&D laboratories, contract research organizations (CROs), and analytical testing facilities engaged in method development, pharmacokinetic studies, and quality control of vitamin D-related products.

Application

• Internal Standard for LC-MS/MS: Critical for the precise quantification of vitamin D2 (ergocalciferol) and its metabolites in biological samples (e.g., serum, plasma) and pharmaceutical formulations.
• Pharmaceutical Research & Development: Used in method validation, stability studies, and metabolic pathway research for vitamin D-based therapeutics.
• Clinical Diagnostics: Supports the development and routine execution of accurate assays for measuring vitamin D status in patient samples.
• Nutraceutical & Food Analysis: Enables reliable testing of vitamin D2 content and fortification levels in dietary supplements and food products.
• Isotope Dilution Mass Spectrometry (IDMS): Serves as the ideal spike for this gold-standard technique, ensuring high metrological traceability in analytical measurements.
• Metabolomics Studies: Facilitates the tracking and quantification of specific vitamin D2 metabolites in complex biological systems.

Basic Information

Product Name	Secalciferol-D6
CAS No.	1440957-55-0
Molecular Formula	C28H38D6O
Molecular Weight	402.66 g/mol
Synonyms	Ergocalciferol-d6; Vitamin D2-d6; (3β,5Z,7E,22E)-9,10-Secocholesta-5,7,10(19),22-tetraen-3-ol-d6; 9,10-Seco(5Z,7E,22E)-5,7,10(19),22-Ergostatetraen-3β-ol-d6; Deuterated Vitamin D2; [²H6]-Ergocalciferol
EINECS	Contact for details

Quality Control

Our Secalciferol-D6 is manufactured under strict quality control protocols to ensure exceptional isotopic purity and chemical integrity, suitable for the most demanding analytical applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing results for critical parameters including isotopic enrichment (D-content), chemical purity by HPLC, and identity confirmation. We adhere to cGMP principles for pharmaceutical-grade reference standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to minimize oxidation. For long-term storage, keep desiccated to prevent moisture absorption. Allow the sealed vial to equilibrate to room temperature before opening to avoid condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline solid or powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to reference standard
Purity (HPLC)	≥ 98.0% (Chemical Purity)
Isotopic Purity (D-content)	≥ 98.0 atom % D
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.