Description

4-Pyridoxic Acid-D3 is a stable isotope-labeled analog of 4-Pyridoxic Acid, a major catabolite of Vitamin B6. This deuterated compound is essential for use as an internal standard in quantitative mass spectrometry, ensuring the highest accuracy and reliability in analytical measurements. It is a critical reagent for researchers and quality control laboratories in the pharmaceutical, clinical diagnostics, and nutritional science industries who require precise quantification of Vitamin B6 metabolites.

Application

• LC-MS/MS Internal Standard: Primary application for the accurate quantification of 4-pyridoxic acid and related Vitamin B6 metabolites in biological matrices (e.g., plasma, serum, urine).
• Clinical Research & Diagnostics: Used in studies investigating Vitamin B6 status, metabolism, and its correlation with various health conditions.
• Pharmaceutical Development: Supports pharmacokinetic (PK) and pharmacodynamic (PD) studies for drugs that interact with or affect Vitamin B6 pathways.
• Nutritional Science & Food Analysis: Enables precise measurement of Vitamin B6 degradation products and metabolite levels in fortified foods and supplements.
• Metabolomics & Biomarker Discovery: A key standard in targeted metabolomics panels for identifying and validating biomarkers related to B-vitamin metabolism.
• Forensic & Toxicology Analysis: Assists in the investigation of cases involving Vitamin B6 deficiency, toxicity, or related metabolic disorders.

Basic Information

Product Name	4-Pyridoxic Acid-D3
CAS No.	1435934-03-4
Molecular Formula	C8H6D3NO4
Molecular Weight	186.17 g/mol
Synonyms	4-Pyridoxic Acid-d3; 5-Pyridoxic Acid-d3; 3-Hydroxy-5-(hydroxymethyl)-2-methylisonicotinic Acid-d3; 2-Methyl-3-hydroxy-4,5-bis(hydroxymethyl)pyridine-d3; Vitamin B6 Catabolite-d3; 4-PA-d3; Deuterated 4-Pyridoxic Acid; [2H3]-4-Pyridoxic Acid
EINECS	Contact for details

Quality Control

Every batch of 4-Pyridoxic Acid-D3 is manufactured and analyzed under strict quality systems to ensure the highest isotopic purity and chemical integrity required for mass spectrometry. Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) detailing isotopic enrichment (D-atom %), chemical purity (by HPLC), and identity confirmation (by NMR/MS) are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under inert atmosphere for long-term stability. The product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent moisture absorption.

Specification

Item	Specification
Appearance	White to Off-white Solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D-atom %)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.