Description

2'-Fluoro-2'-Deoxy-Ara-C-3'-Phosphoramidite is a high-purity, protected nucleoside phosphoramidite monomer, a critical building block for the solid-phase synthesis of oligonucleotides. Its core value lies in enabling the precise and efficient incorporation of a modified cytidine analog into synthetic DNA/RNA strands, which is essential for advanced research and therapeutic development. This compound is primarily needed by biotechnology and pharmaceutical companies engaged in antisense therapy, siRNA development, diagnostic probe synthesis, and fundamental nucleic acid research.

Application

• Antisense Oligonucleotide (ASO) Synthesis: For the production of therapeutic oligonucleotides targeting specific mRNA sequences.
• Small Interfering RNA (siRNA) Manufacture: As a key monomer in constructing siRNA strands for gene silencing applications.
• Diagnostic Probe and Primer Development: Used to create fluorescently labeled or otherwise modified probes for PCR, FISH, and microarray technologies.
• Fundamental Nucleic Acid Research: Enables the study of structure-activity relationships (SAR) by introducing fluorine modifications at the 2' sugar position.
• Aptamer Discovery: For the synthesis of modified DNA libraries in SELEX processes to develop high-affinity binding molecules.
• Therapeutic Oligonucleotide Scale-Up: Supports GMP-grade production for clinical and commercial-stage drug candidates.

Basic Information

Product Name	2'-Fluoro-2'-Deoxy-Ara-C-3'-Phosphoramidite
CAS No.	1404463-12-2
Molecular Formula	C34H43FN5O7P
Molecular Weight	683.71 g/mol
Synonyms	2'-F-ara-C Phosphoramidite; 1-[(2R,3R,4R,5R)-5-[Bis(4-methoxyphenyl)-phenylmethoxy]methyl-4-fluoro-3-(4-methoxyphenoxy)oxolan-2-yl]-5-fluoro-2-oxopyrimidin-1-ium-3-yl 2-cyanoethyl diisopropylphosphoramidite; 5'-O-DMT-2'-F-2'-deoxy-ara-C-3'-CE Phosphoramidite; 2'-Fluoro-arabinocytidine-3'-phosphoramidite; N4-Benzoyl-2'-fluoro-2'-deoxy-arabinocytidine-3'-phosphoramidite (common protected form); 2'-F-ara-CE Phosphoramidite; 2'-Fluoro-2'-deoxy-arabinofuranosylcytosine 3'-phosphoramidite
EINECS	Contact for details

Quality Control

Our 2'-Fluoro-2'-Deoxy-Ara-C-3'-Phosphoramidite is manufactured under strict quality systems to ensure batch-to-batch consistency for sensitive oligonucleotide synthesis. Each lot undergoes comprehensive analytical testing, including HPLC purity assay, 31P and 1H/19F NMR for structural confirmation, and moisture content (Karl Fischer) analysis. Certificates of Analysis (COA) with full specification data are provided and can be tailored to meet specific project or regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C under an inert atmosphere (argon or nitrogen). This product is strictly light-sensitive (protect from all light exposure), easily oxidized (store under inert atmosphere), and hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to reference spectrum
Purity (HPLC)	≥ 97.0%
Moisture Content (KF)	≤ 1.0%
Solubility	Soluble in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.