Description

Propargyl-Peg3-1-o-(B-Cyanoethyl-N,n-Diisopropyl)Phosphoramidite is a specialized phosphoramidite monomer, a critical building block in the automated synthesis of oligonucleotides. This compound is engineered to introduce a propargyl group via a triethylene glycol (PEG3) linker, enabling subsequent bioorthogonal "click" chemistry modifications such as the copper-catalyzed azide-alkyne cycloaddition (CuAAC). It is essential for researchers and manufacturers in pharmaceutical development, diagnostic probe synthesis, and advanced materials science who require precise, site-specific functionalization of nucleic acids.

Application

• Oligonucleotide Synthesis: Key monomer for the solid-phase synthesis of modified DNA/RNA strands with alkyne handles.
• Probe and Primer Labeling: Enables the attachment of fluorophores, biotin, quenchers, or other reporter molecules via post-synthetic click chemistry.
• Therapeutic Oligonucleotide Development: Used in the research and production of antisense oligonucleotides (ASOs), siRNA, and aptamers requiring conjugation.
• DNA-Encoded Library (DEL) Technology: Facilitates the creation of diverse chemical libraries by tagging DNA barcodes with small molecules.
• Surface Immobilization: For functionalizing chips, beads, or nanoparticles with oligonucleotides for biosensing and diagnostic applications.
• Materials Science: Building block for creating DNA-based nanostructures and hybrid organic-inorganic materials.

Basic Information

Item	Detail
Product Name	Propargyl-Peg3-1-o-(B-Cyanoethyl-N,n-Diisopropyl)Phosphoramidite
CAS No.	1391728-01-0
Molecular Formula	C22H34N3O5P
Molecular Weight	451.50 g/mol
Synonyms	Propargyl-PEG3-phosphoramidite; 5'-O-(4,4'-Dimethoxytrityl)-2'-deoxy-5-propargylaminoallyluridine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite (related structural analog); Alkyne-PEG3-phosphoramidite; 1-O-(2-Cyanoethoxy-diisopropylaminophosphino)-3-O-(propargyl)-triethyleneglycol; Propargyl triethylene glycol phosphoramidite; 5'-Propargyl-PEG3 Phosphoramidite; Click Chemistry Phosphoramidite Monomer
EINECS	Contact for details

Quality Control

Every batch of our phosphoramidite monomers is produced and tested under strict inert atmosphere conditions to ensure stability and performance. We provide comprehensive Certificates of Analysis (COA) detailing purity, identity, and moisture content, with specifications tailored to meet the rigorous demands of GMP-compliant oligonucleotide manufacturing. Our quality systems support traceability and consistency for critical research and production applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and easily oxidized; prolonged exposure to air, moisture, or elevated temperatures will lead to decomposition and reduced coupling efficiency.

Specification

Item	Specification
Appearance	Colorless to pale yellow viscous oil or solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 0.5%
Phosphitylation Efficiency	≥ 98% (by controlled pore glass test)
Solubility	Soluble in anhydrous acetonitrile, dichloromethane, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.