Description

Dbco-C6-Nhs Ester CAS NO 1384870-47-6 is a heterobifunctional crosslinking reagent featuring a dibenzocyclooctyne (DBCO) group for strain-promoted azide-alkyne cycloaddition (SPAAC) and an N-hydroxysuccinimide (NHS) ester for amine conjugation. This compound is critical for creating stable, catalyst-free bioconjugates, enabling efficient and specific labeling of biomolecules without cytotoxic copper catalysts. It is essential for researchers and manufacturers in bioconjugation, antibody-drug conjugate (ADC) development, proteomics, and the synthesis of diagnostic and therapeutic agents, where reliable, high-purity linkers are paramount.

Application

• Antibody-Drug Conjugate (ADC) Linker-Payload Synthesis: For the stable, site-specific conjugation of cytotoxic drugs to monoclonal antibodies.
• Fluorescent & Biotin Probe Labeling: Enables the creation of detection reagents for imaging, flow cytometry, and ELISA applications via copper-free click chemistry.
• Protein & Peptide Modification: Used to functionalize amines on proteins, peptides, and enzymes with DBCO handles for subsequent bioorthogonal labeling.
• Surface Functionalization & Material Science: For immobilizing biomolecules onto azide-modified surfaces, nanoparticles, or hydrogels to create bioactive materials.
• Oligonucleotide Conjugation: Facilitates the labeling of amine-modified DNA, RNA, or oligonucleotide analogs for genetic analysis and therapeutic platforms.
• Polymer & Dendrimer Chemistry: Serves as a key building block for synthesizing multifunctional polymers with precise bio-orthogonal reactivity.
• Diagnostic Kit Development: Integral in developing sensitive immunoassays and molecular diagnostic tools requiring stable reporter linkages.
• Pre-Targeting Strategies in Radioimmunotherapy: Used in multi-step pretargeting approaches to improve tumor targeting and reduce off-target radiation exposure.

Basic Information

Product Name	Dbco-C6-Nhs Ester
CAS No.	1384870-47-6
Molecular Formula	C30H30N2O6
Molecular Weight	514.57 g/mol
Synonyms	DBCO-C6-NHS ester; DBCO-SE; Dibenzocyclooctyne-C6-NHS ester; (1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl (6-((2,5-dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)carbamate; 6-((((1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethoxy)carbonyl)amino)hexanoic acid N-hydroxysuccinimide ester; DBCO-amine linker NHS ester; DBCO-C6-NHS; Azadibenzocyclooctyne-C6-NHS ester
EINECS	Contact for details

Quality Control

Our Dbco-C6-Nhs Ester is manufactured under strict quality management systems. Every batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry for identity verification, to ensure it meets the high standards required for sensitive bioconjugation research and cGMP-guided therapeutic development. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee traceability, consistency, and compliance with your research or production protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with reference structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.