Description

5-(Methyl-D3)Tetrahydrofolic Acid (Mixture Of Diastereomers) is a stable isotope-labeled analog of the biologically active form of folic acid, where three hydrogen atoms are replaced by deuterium at the methyl group. This compound is critical for advanced research and development in pharmaceutical and analytical chemistry, enabling precise metabolic and pharmacokinetic studies. It is primarily sought by research institutions and pharmaceutical companies engaged in the development of folate-based therapeutics, clinical diagnostics, and stable isotope dilution mass spectrometry (SID-MS) methods.

Application

• Internal Standard for LC-MS/MS Analysis: Serves as a critical quantitative reference standard in bioanalytical methods for measuring folate levels in biological matrices like plasma, serum, and tissues.
• Pharmaceutical R&D: Used in the development and testing of novel antifolate drugs, cancer therapeutics, and metabolic pathway modulators.
• Clinical Research & Diagnostics: Enables accurate quantification of folate and its metabolites in clinical studies related to nutrition, pregnancy, and metabolic disorders.
• Metabolic Tracing Studies: Acts as a tracer to investigate one-carbon metabolism, DNA synthesis, and methylation processes in cell culture and animal models.
• Reference Material Manufacturing: Used in the production of certified reference materials (CRMs) and quality control standards for clinical diagnostic kits.
• Nutraceutical Research: Supports studies on folate bioavailability, stability, and formulation in fortified foods and dietary supplements.

Basic Information

Product Name	5-(Methyl-D3)Tetrahydrofolic Acid (Mixture Of Diastereomers)
CAS No.	1356019-97-0
Molecular Formula	C20H22D3N7O6
Molecular Weight	462.46 g/mol
Synonyms	(6S)-5-Methyl-5,6,7,8-tetrahydrofolic Acid-d3; 5-CH3-THF-d3; L-Methyltetrahydrofolate-d3; (6S)-5-Methyltetrahydrofolate-d3; 5-Methyl-(6S)-tetrahydrofolate-d3; Deuterated 5-Methyltetrahydrofolic Acid; 5-Methyl-THF-d3; 5-MTHF-d3
EINECS	Contact for details

Quality Control

Our 5-(Methyl-D3)Tetrahydrofolic Acid is manufactured under controlled conditions to ensure high chemical purity and isotopic enrichment. Each batch is subjected to rigorous analytical testing, including HPLC for chemical purity, NMR and MS for structural confirmation and deuterium incorporation, and residual solvent analysis. A comprehensive Certificate of Analysis (COA) detailing purity, assay, isotopic purity, and specific tests is provided to guarantee consistency and reliability for your critical research applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to ensure long-term stability. The material is hygroscopic (moisture-sensitive) and must be handled in a dry environment to prevent degradation.

Specification

Item	Specification
Appearance	Yellow to orange powder or solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Isotopic Purity (D3)	≥ 98.0 atom % D
Chemical Purity (NMR)	Conforms
Water Content (KF)	≤ 5.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.