Description

n-Fmoc-L-Phenylalanine-D8 CAS NO 1353853-39-0 is a deuterated derivative of the protected amino acid Fmoc-L-phenylalanine, where eight hydrogen atoms are replaced by deuterium, a stable, non-radioactive isotope. This high isotopic purity is critical for applications requiring precise mass spectrometry analysis and metabolic studies, offering enhanced sensitivity and reduced background noise. It is an essential building block for researchers and manufacturers in pharmaceutical development, proteomics, and stable isotope labeling, particularly in the synthesis of deuterated peptides for use as internal standards in quantitative LC-MS/MS assays.

Application

• Internal Standard for Quantitative Bioanalysis: Used as a critical reference standard in Liquid Chromatography-Mass Spectrometry (LC-MS/MS) for the accurate quantification of L-phenylalanine and related peptides in complex biological matrices like plasma and tissue samples.
• Deuterated Peptide Synthesis: Serves as a key protected amino acid precursor in solid-phase peptide synthesis (SPPS) for creating isotopically labeled peptides used in drug metabolism and pharmacokinetics (DMPK) studies.
• Metabolic Pathway Tracing: Employed in metabolic flux analysis and proteomics research to track and quantify biochemical pathways and protein turnover rates.
• Pharmaceutical Reference Standard: Provides a certified standard for method development, validation, and quality control in pharmaceutical laboratories to ensure assay accuracy and regulatory compliance.
• Proteomics and Biomarker Discovery: Facilitates relative and absolute quantification in shotgun proteomics, aiding in the identification and validation of disease biomarkers.
• Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC): Can be utilized in advanced cell culture techniques for comparative proteomic analysis.

Basic Information

Product Name	n-Fmoc-L-Phenylalanine-D8
CAS No.	1353853-39-0
Molecular Formula	C24H13D8NO4
Molecular Weight	399.46 g/mol
Synonyms	(2S)-2-[(9H-Fluoren-9-ylmethoxy)carbonyl]amino-3-(phenyl-d5)propanoic-3,3-d2 acid; Fmoc-Phe(D8)-OH; N-[(9H-Fluoren-9-ylmethoxy)carbonyl]-L-phenylalanine-d8; Fmoc-L-Phenylalanine-d8; N-α-Fmoc-L-phenylalanine-d8; (2S)-2-[[(9H-Fluoren-9-ylmethoxy)carbonyl]amino]-3-(2,3,4,5,6-pentadeuteriophenyl)propanoic-3,3-d2 acid; Deuterated Fmoc-Phenylalanine
EINECS	Contact for details

Quality Control

Our n-Fmoc-L-Phenylalanine-D8 is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for chemical and isotopic purity, NMR for structural confirmation, and MS for identity verification, to ensure it meets the high standards required for research and analytical applications. A comprehensive Certificate of Analysis (COA) detailing batch-specific results for assay, deuterium enrichment, and impurities is provided to guarantee traceability and consistency.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This compound is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and purity. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (Deuterium Enrichment)	≥ 98 atom % D
Specific Optical Rotation	Contact for details
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.