Description

[2H3]-3’-Deoxy-3’-Fluorothymidine CAS NO 1346523-18-9 is a deuterium-labeled isotopologue of the nucleoside analogue 3’-Deoxy-3’-Fluorothymidine (FLT), a critical compound used as a biomarker for cellular proliferation. This stable isotope-labeled version is essential for precise pharmacokinetic and metabolic studies, providing unmatched accuracy in quantitative mass spectrometry analysis. It is primarily utilized by pharmaceutical R&D teams, clinical research organizations, and diagnostic developers working on oncology and antiviral therapeutics.

Application

• Internal Standard for LC-MS/MS: Serves as a crucial quantitative internal standard in bioanalytical assays for the parent drug FLT, ensuring accurate measurement of drug concentrations in biological matrices like plasma and tissue.
• Drug Metabolism and Pharmacokinetics (DMPK) Studies: Enables detailed investigation of absorption, distribution, metabolism, and excretion (ADME) profiles in preclinical and clinical development.
• Biomarker Research: Used in the development and validation of assays for thymidine kinase 1 (TK1) activity, a biomarker for cellular proliferation in cancer.
• Positron Emission Tomography (PET) Tracer Validation: Supports the development and calibration of [18F]FLT, a widely used PET imaging agent for tumor proliferation.
• Stable Isotope Tracing Experiments: Facilitates advanced metabolic flux analysis to study nucleotide synthesis pathways in cell cultures.
• Reference Standard for Quality Control: Acts as a certified reference material for the pharmaceutical quality control of FLT-based active pharmaceutical ingredients (APIs).

Basic Information

Product Name	[2H3]-3’-Deoxy-3’-Fluorothymidine
CAS No.	1346523-18-9
Molecular Formula	C10H11D3FN2O5
Molecular Weight	264.23 g/mol
Synonyms	3'-Deoxy-3'-[18F]fluorothymidine-d3; FLT-d3; Alovudine-d3; 1-((2R,4S,5R)-4-Fluoro-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methyl-2,4(1H,3H)-pyrimidinedione-d3; 3'-Fluoro-3'-deoxythymidine-d3; Deuterated FLT; [D3]-FLT; 3’-Deoxy-3’-fluorothymidine (trideuterated)
EINECS	Contact for details

Quality Control

Every batch of [2H3]-3’-Deoxy-3’-Fluorothymidine is manufactured under strict quality management systems and undergoes comprehensive analytical characterization. We guarantee high chemical and isotopic purity, supported by a detailed Certificate of Analysis (COA) that includes data from NMR (1H, 19F), mass spectrometry, and HPLC. Our standards align with the requirements for pharmaceutical reference materials, ensuring reliability for your most sensitive analytical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term storage under inert atmosphere, please contact us.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms
Purity (HPLC)	≥ 98.0%
Isotopic Purity (MS)	≥ 98.0 atom % D
Chemical Purity	≥ 95.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.