Description

4-Methoxy-D3-17B-Estradiol is a deuterated analog of a key estrogenic steroid, specifically designed for advanced research and analytical applications. This compound is crucial for use as an internal standard in mass spectrometry, enabling highly accurate quantification and metabolic studies of estradiol derivatives. It is primarily needed by pharmaceutical R&D laboratories, clinical research organizations, and analytical service providers focused on endocrinology, drug metabolism, and bioanalytical method development.

Application

• Internal Standard for LC-MS/MS: Essential for the precise quantification of endogenous estradiol and its metabolites in complex biological matrices like plasma, serum, and tissue homogenates.
• Drug Metabolism and Pharmacokinetics (DMPK) Studies: Used in tracing metabolic pathways, assessing bioavailability, and studying the pharmacokinetic profile of estrogen-based therapeutics.
• Clinical Research and Diagnostics: Supports the development and validation of sensitive assays for hormonal level monitoring in endocrinological disorders.
• Isotope Dilution Mass Spectrometry (IDMS): Serves as a critical reference material in high-precision analytical techniques for achieving superior accuracy and reproducibility.
• Biomedical Research: Facilitates in-vitro and in-vivo studies investigating estrogen receptor signaling, hormone-dependent cancers, and endocrine-disrupting compounds.
• Pharmaceutical Quality Control: Employed in the analysis and stability testing of steroid-based pharmaceutical products to ensure potency and purity.

Basic Information

Product Name	4-Methoxy-D3-17B-Estradiol
CAS No.	1334143-12-2
Molecular Formula	C19H21D3O3
Molecular Weight	303.41 g/mol
Synonyms	17β-Estradiol-2,4,16,16,17-d5 3-Methyl Ether; Deuterated 4-Methoxyestradiol; 4-MeO-E2-d3; 4-Methoxyestra-1,3,5(10)-triene-3,17β-diol-2,4,16,16,17-d5 3-Methyl Ether; 3-Methoxy-17β-hydroxy-1,3,5(10)-estratriene-2,4,16,16,17-d5; Estra-1,3,5(10)-triene-3,17β-diol-2,4,16,16,17-d5, 3-methyl ether
EINECS	Contact for details

Quality Control

Our 4-Methoxy-D3-17B-Estradiol is manufactured under strict quality systems to ensure the high chemical and isotopic purity required for sensitive analytical applications. Each batch is characterized by advanced spectroscopic and chromatographic methods, including NMR and HPLC-MS, to confirm identity, deuterium incorporation, and purity. A comprehensive Certificate of Analysis (COA) detailing isotopic enrichment, chemical purity, and analytical results is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Purity (HPLC)	≥ 98.0%
Isotopic Purity (MS)	≥ 98.0 atom % D
Chemical Purity (NMR)	Conforms
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.