Description

3-[n-Acetyl-L-Cystein-S-Yl] Acetaminophen-D5 Sodium Salt is a high-purity, deuterated internal standard critical for advanced analytical chemistry and pharmaceutical research. This compound is specifically designed for use in mass spectrometry to ensure accurate quantification and method validation of acetaminophen and its metabolites in complex biological matrices. It is an essential tool for researchers and quality control laboratories in the pharmaceutical, clinical diagnostics, and forensic toxicology sectors who require the highest standards of precision and reliability in their analytical workflows.

Application

• Internal Standard for LC-MS/MS: Primary application for the precise quantification of acetaminophen (paracetamol) and its cysteine conjugate in pharmacokinetic, bioequivalence, and toxicology studies.
• Pharmaceutical R&D: Used in drug metabolism and pharmacokinetics (DMPK) studies to track the metabolic pathway of acetaminophen and investigate potential hepatotoxicity mechanisms.
• Clinical Research: Enables accurate measurement of drug and metabolite levels in patient plasma, serum, or urine for therapeutic drug monitoring and overdose cases.
• Forensic Analysis: Provides definitive identification and quantification in post-mortem and legal toxicology investigations.
• Method Development & Validation: Serves as a critical reference material for developing, optimizing, and validating robust analytical methods in compliance with FDA, EMA, and ICH guidelines.
• Biomarker Research: Facilitates studies on oxidative stress and glutathione depletion by accurately measuring specific acetaminophen-protein adducts.

Basic Information

Product Name	3-[n-Acetyl-L-Cystein-S-Yl] Acetaminophen-D5 Sodium Salt
CAS No.	1331911-02-4
Molecular Formula	C13H10D5N2NaO5S
Molecular Weight	345.36 g/mol (approx.)
Synonyms	APAP-CYS-D5 Sodium Salt; N-Acetyl-p-benzoquinone imine-Cysteine Adduct-D5 Sodium Salt; NAPQI-CYS-D5 Sodium Salt; 3-(N-Acetyl-L-cystein-S-yl)-acetaminophen-d5 Sodium Salt; Acetaminophen Cysteine Adduct-d5 Sodium Salt; Paracetamol Cysteine Conjugate-d5 Sodium Salt; 4'-Hydroxyacetanilide-3,5-d2, N-acetyl-d3 Cysteine Adduct Sodium Salt
EINECS	Contact for details

Quality Control

Every batch of 3-[n-Acetyl-L-Cystein-S-Yl] Acetaminophen-D5 Sodium Salt is manufactured and analyzed under strict quality systems to ensure it meets the exacting requirements for use as an internal standard. Our products undergo rigorous quality testing, including advanced spectroscopic and chromatographic techniques, to guarantee high chemical purity, isotopic enrichment, and structural integrity. Certificates of Analysis (COA) detailing batch-specific results for identity, purity, and isotopic abundance are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC-MS)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Isotopic Purity (Deuterium Enrichment)	≥ 98 atom % D
Water Content (KF)	≤ 5.0 %
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.