Description

Nε-(Ethoxycarbonylmethyl)-L-Lysine-D4 Methyl Ester is a stable isotope-labeled derivative of L-lysine, specifically deuterated at four positions. This compound is a critical building block in the synthesis of isotopically labeled peptides and proteins for advanced research applications. It is primarily utilized by pharmaceutical R&D teams and analytical laboratories engaged in quantitative proteomics, metabolic pathway tracing, and pharmacokinetic studies.

Application

• Internal Standard for LC-MS/MS Analysis: Serves as a high-purity, isotopically labeled internal standard for the accurate quantification of lysine and its derivatives in complex biological matrices.
• Proteomics & Metabolomics Research: Used in the synthesis of deuterium-labeled peptides for mass spectrometry-based protein quantification and metabolic flux analysis.
• Drug Metabolism and Pharmacokinetics (DMPK): Essential for tracing the absorption, distribution, metabolism, and excretion (ADME) of drug candidates containing lysine moieties.
• Biomarker Discovery and Validation: Enables precise measurement of lysine-related biomarkers in disease research, improving assay accuracy and reliability.
• Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC): A key reagent for metabolic labeling in cell culture to study dynamic protein expression.
• Synthesis of Labeled Biologics: Acts as a precursor in the chemical or enzymatic synthesis of isotopically labeled antibodies, enzymes, and other therapeutic proteins.

Basic Information

Product Name	Nε-(Ethoxycarbonylmethyl)-L-Lysine-D4 Methyl Ester
CAS No.	1331910-52-1
Molecular Formula	C11H16D4N2O4
Molecular Weight	248.31 g/mol
Synonyms	L-Lysine, N6-[(ethoxycarbonyl)methyl]-, methyl ester, d4; (2S)-6-Amino-2-[(ethoxycarbonyl)methyl]amino]hexanoic acid methyl ester-d4; Methyl Nε-(ethoxycarbonylmethyl)-L-lysinate-d4; Deuterated Boc-Lys(Alloc)-OMe analog; Nε-Carbethoxymethyl-L-lysine methyl ester-d4; [2H4]-Nε-(Ethoxycarbonylmethyl)lysine methyl ester; Stable isotope-labeled lysine derivative
EINECS	Contact for details

Quality Control

Our Nε-(Ethoxycarbonylmethyl)-L-Lysine-D4 Methyl Ester is manufactured under strict quality systems to ensure the high chemical and isotopic purity required for sensitive analytical applications. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing parameters such as isotopic enrichment (D, atom %), chemical purity by HPLC, and chiral purity. We adhere to relevant industry guidelines for the production of stable isotope-labeled compounds.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive). For long-term storage, consider storing under an inert atmosphere to maintain stability and isotopic integrity.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment (D)	≥ 98 atom % D
Chiral Purity (HPLC)	≥ 99.0% (L-isomer)
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.