Description

4-Aminophenazone-D3 is a deuterium-labeled analog of the pharmaceutical compound aminophenazone, specifically designed for use as an internal standard in quantitative analytical methods. This high-purity stable isotope-labeled compound is essential for ensuring accuracy and reliability in mass spectrometry-based analyses, particularly in pharmacokinetic and metabolic studies. It is primarily utilized by pharmaceutical researchers, contract research organizations (CROs), and analytical laboratories engaged in drug development and bioanalytical method validation.

Application

• Internal Standard for LC-MS/MS: Serves as a critical internal standard for the precise quantification of aminophenazone and its metabolites in biological matrices like plasma, serum, and urine.
• Drug Metabolism and Pharmacokinetics (DMPK) Studies: Enables accurate tracking of drug absorption, distribution, metabolism, and excretion (ADME) in preclinical and clinical research.
• Bioanalytical Method Development and Validation: Used to develop robust, sensitive, and specific analytical methods compliant with FDA, EMA, and ICH guidelines.
• Pharmaceutical Impurity Profiling: Aids in the identification and quantification of related substances and degradation products in active pharmaceutical ingredient (API) batches.
• Forensic and Clinical Toxicology: Supports accurate measurement of aminophenazone in cases of overdose or therapeutic drug monitoring.
• Isotope Dilution Mass Spectrometry (IDMS): Employed in high-accuracy reference measurement procedures to minimize matrix effects and ionization variability.

Basic Information

Product Name	4-Aminophenazone-D3
CAS No.	1329792-51-9
Molecular Formula	C13H14D3N3O
Molecular Weight	234.31 g/mol
Synonyms	Aminophenazone-d3; 4-Aminoantipyrine-d3; 4-Amino-2,3-dimethyl-1-phenyl-3-pyrazolin-5-one-d3; 1-Phenyl-2,3-dimethyl-4-amino-5-pyrazolone-d3; Deuterated Aminophenazone; [2H3]-4-Aminophenazone; Ampyrone-d3; Pyramidon-d3
EINECS	Contact for details

Quality Control

Every batch of 4-Aminophenazone-D3 is manufactured and tested under strict quality management systems to ensure exceptional isotopic purity and chemical integrity. Our products undergo rigorous quality testing, including advanced spectroscopic and chromatographic analyses, to ensure compliance with the stringent requirements for stable isotope-labeled reference materials. A comprehensive Certificate of Analysis (COA), detailing isotopic enrichment (D-enrichment), chemical purity, and analytical results, is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to minimize exposure to atmospheric humidity. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D-enrichment)	≥ 98 atom % D
Chemical Purity (NMR)	Conforms
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.