Description

3-Demethyl Colchicine-D3 is a deuterated analog of a key colchicine derivative, serving as a critical stable isotope-labeled internal standard for precise analytical quantification. This high-purity reference material is essential for ensuring accuracy and reliability in sophisticated bioanalytical and pharmacokinetic studies. It is primarily utilized by pharmaceutical R&D laboratories, contract research organizations (CROs), and academic institutions engaged in advanced metabolic research and drug development programs for anti-inflammatory and anti-cancer agents.

Application

• Internal Standard for LC-MS/MS: Primary use as a deuterium-labeled internal standard in Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) for the accurate quantification of colchicine and its metabolites in complex biological matrices.
• Pharmacokinetic & Metabolic Studies: Enables precise tracking of drug absorption, distribution, metabolism, and excretion (ADME) profiles in preclinical and clinical research.
• Bioanalytical Method Development & Validation: Critical for developing, optimizing, and validating robust analytical methods in compliance with regulatory guidelines (e.g., FDA, EMA).
• Drug Impurity Profiling: Used as a reference compound to identify and quantify related substances and degradation products in active pharmaceutical ingredient (API) batches.
• Isotope Dilution Mass Spectrometry (IDMS): Employed in high-precision IDMS techniques to achieve superior accuracy in quantitative analysis, minimizing matrix effects.
• Mechanistic Biochemical Research: Supports fundamental research into tubulin binding dynamics and the mechanistic pathways of colchicine-related compounds.

Basic Information

Product Name	3-Demethyl Colchicine-D3
CAS No.	1314417-96-3
Molecular Formula	C₂₁H₂₀D₃NO₆
Molecular Weight	~386.42 g/mol
Synonyms	N-Deacetyl-N-(2,3,4-trimethoxybenzoyl)-3-demethylcolchicine-d3; Colchicine-d3 derivative; 3-Demethylcolchicine D3; Deuterated Colchicine Metabolite Standard; (S)-N-(5,6,7,9-Tetrahydro-1,2,3,10-tetramethoxy-9-oxobenzo[a]heptalen-7-yl)-2,3,4-trimethoxybenzamide-d3; N-[(7S)-5,6,7,9-Tetrahydro-1,2,3,10-tetramethoxy-9-oxobenzo[a]heptalen-7-yl]-2,3,4-trimethoxybenzamide-d3
EINECS	Contact for details

Quality Control

Every batch of 3-Demethyl Colchicine-D3 is manufactured and analyzed under strict quality systems. We guarantee high chemical and isotopic purity suitable for sensitive analytical applications. Comprehensive quality control includes identity confirmation, assay determination, and impurity profiling via advanced chromatographic and spectroscopic techniques. Certificates of Analysis (COA) detailing batch-specific results for purity, isotopic enrichment, and related substances are provided and available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to minimize exposure to atmospheric humidity. For long-term storage, consider desiccants or inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D3)	≥ 98 atom % D
Chemical Purity	≥ 95%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.