Description

(+/-)-4-Hydroxy-2E-Nonenal-D3 CAS NO 1313400-91-7 is a deuterium-labeled analog of 4-Hydroxy-2-nonenal (4-HNE), a key secondary product of lipid peroxidation. This stable isotope-labeled compound is an essential internal standard for the precise quantification of 4-HNE in complex biological matrices using mass spectrometry. It is primarily required by researchers and analytical laboratories in the fields of oxidative stress research, lipidomics, biomarker discovery, and toxicology to ensure accurate and reliable analytical results.

Application

• Internal Standard for Mass Spectrometry (MS): Critical for quantitative LC-MS/MS and GC-MS analysis of endogenous 4-HNE levels in biological samples (plasma, tissue, cells).
• Biomarker Research: Enables accurate measurement of 4-HNE as a biomarker for oxidative stress and lipid peroxidation in disease studies (e.g., neurodegenerative diseases, cardiovascular disorders, cancer).
• Metabolic Pathway Tracing: Used in stable isotope dilution assays (SIDA) to study the metabolism and adduct formation of 4-HNE with proteins and DNA.
• Pharmaceutical & Nutraceutical R&D: Supports the development and efficacy testing of antioxidants and therapeutic agents targeting oxidative damage.
• Food & Nutritional Science: Applied in research on lipid oxidation in food products and the impact of dietary components on oxidative stress markers.
• Toxicology Studies: Facilitates the assessment of chemical-induced oxidative stress and cellular damage.
• Quality Control for Diagnostic Kits: Serves as a reference standard in the calibration and validation of commercial assay kits for oxidative stress markers.

Basic Information

Item	Details
Product Name	(+/-)-4-Hydroxy-2E-Nonenal-D3
CAS No.	1313400-91-7
Molecular Formula	C9H11D3O2
Molecular Weight	157.23 g/mol
Synonyms	4-Hydroxy-2-nonenal-d3; 4-HNE-d3; HNE-d3; (E)-4-Hydroxynon-2-enal-d3; 4-Hydroxy-(2E)-nonenal-d3; 4-Hydroxynonenal-d3; Lipid peroxidation product-d3; Deuterated 4-HNE
EINECS	Contact for details

Quality Control

Our (+/-)-4-Hydroxy-2E-Nonenal-D3 is produced and handled under strict quality protocols to ensure the high chemical purity and isotopic enrichment required for sensitive analytical applications. Each batch is characterized by advanced spectroscopic and chromatographic techniques. Certificates of Analysis (COA) detailing purity, isotopic purity (D-enrichment), and analytical data are provided to guarantee traceability and compliance with your research standards.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below under an inert atmosphere. The compound is moisture-sensitive and should be handled in a dry environment. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation.

Specification

Item	Specification
Appearance	Colorless to pale yellow liquid / oil
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95% (Area %)
Isotopic Purity (MS)	≥ 98 atom % D
Chemical Purity (GC/MS or LC-MS)	Conforms
Solubility	Soluble in ethanol, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.